| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,821,628 | G→A | 57.7% | G266G (GGC→GGT) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,821,628 | 0 | G | A | 57.7% | 6.3 / 29.0 | 26 | G266G (GGC→GGT) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base G (5/6); new base A (10/5); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 | |||||||||||
CTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTT > W3110S.gb/2821568‑2821689 | ctcGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTCATACCTTCACCGTAGAgg < 1:3343030/70‑1 (MQ=255) gATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGa < 1:2228329/68‑1 (MQ=255) tCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTAGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTgg < 1:3446991/71‑1 (MQ=255) aGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGa < 1:1918843/70‑1 (MQ=255) tctcTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAAtt > 1:1131498/1‑69 (MQ=255) tctTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGc > 1:1024684/1‑71 (MQ=255) tctTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGc > 1:1899659/1‑71 (MQ=255) cGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTa < 1:2855043/71‑1 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTaga > 1:896399/1‑39 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTaga > 1:3498708/1‑39 (MQ=255) cAACCAGTTCGCCGTAGAAGTTGATACCTTCACCGTaga > 1:1567502/1‑39 (MQ=255) cGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTg > 1:2604132/1‑71 (MQ=255) cGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTg > 1:2918582/1‑71 (MQ=255) cGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTg > 1:418153/1‑71 (MQ=255) cGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTg > 1:2191425/1‑71 (MQ=255) cGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTg > 1:681737/1‑71 (MQ=255) cGCCGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTg > 1:1285534/1‑71 (MQ=255) ccGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGtt < 1:2303746/71‑1 (MQ=255) ccGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGtt < 1:1749550/71‑1 (MQ=255) ccGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGtt < 1:1470179/71‑1 (MQ=255) cGTAGAAGTTGATACCTTCACCGTAGAGGATCTGTAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGttc < 1:2967948/71‑1 (MQ=255) cGTAGAAGTTGATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGttc < 1:3042327/71‑1 (MQ=255) ttGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcaccac < 1:182857/71‑1 (MQ=255) gATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACtt > 1:2711647/1‑71 (MQ=255) gATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACtt > 1:1671731/1‑71 (MQ=255) gATACCTTCACCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAttt > 1:440237/1‑56 (MQ=255) | CTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTT > W3110S.gb/2821568‑2821689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |