| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,840,761 | A→G | 89.8% | E372E (GAA→GAG) | ascB → | cryptic 6‑phospho‑beta‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,840,761 | 0 | A | G | 89.8% | 176.0 / 8.8 | 59 | E372E (GAA→GAG) | ascB | cryptic 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (26/27); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCA > W3110S.gb/2840698‑2840822 | cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGcc > 1:3175167/1‑71 (MQ=255)cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGcc > 1:1306599/1‑71 (MQ=255)cGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGc > 1:3085762/1‑70 (MQ=255) tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGTCCTGGGCGCaa < 1:989601/71‑1 (MQ=255) tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCaa < 1:3058619/71‑1 (MQ=255) tATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCaa < 1:664937/71‑1 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGagaa > 1:905480/1‑53 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCaa > 1:476304/1‑67 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCaa > 1:1199818/1‑67 (MQ=255) aCCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCaa > 1:551438/1‑67 (MQ=255) aTGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGACGCAAAAGATg > 1:3015769/1‑70 (MQ=255) tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGaa < 1:250722/71‑1 (MQ=255) tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGTGCGCAAAAGATGaa < 1:647456/71‑1 (MQ=255) tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa < 1:3694141/71‑1 (MQ=255) tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa < 1:3537263/71‑1 (MQ=255) tGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGaa < 1:925862/71‑1 (MQ=255) aaTATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAtt > 1:2412432/1‑71 (MQ=255) aaTATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAtt > 1:330174/1‑71 (MQ=255) tgatgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:263449/1‑66 (MQ=255) tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2229636/71‑1 (MQ=255) tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3113706/71‑1 (MQ=255) tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3312889/71‑1 (MQ=255) tgTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:143052/71‑1 (MQ=255) gTACGTTCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1424907/70‑1 (MQ=255) gTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:2748207/1‑70 (MQ=255) gTACGATAGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc > 1:1241810/1‑70 (MQ=255) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa < 1:3060360/71‑1 (MQ=255) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa < 1:2890892/71‑1 (MQ=255) tACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa < 1:2081117/71‑1 (MQ=255) cGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGc > 1:655412/1‑69 (MQ=255) cGTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGc > 1:2568758/1‑69 (MQ=255) gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa > 1:2875847/1‑64 (MQ=255) gTTATCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2292421/70‑1 (MQ=255) gTTATAAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCaa > 1:2715679/1‑64 (MQ=255) aTCAGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGAt > 1:3046835/1‑70 (MQ=255) aGAAGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAac > 1:170270/1‑71 (MQ=255) aaGCCGCTGTTTCTGGTGGAGAACGGCCTTGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacga > 1:3588274/1‑71 (MQ=255) aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCga < 1:2071026/62‑1 (MQ=255) aaGCCGCTGTTTCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAacga > 1:1100832/1‑71 (MQ=255) tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGa < 1:941928/35‑1 (MQ=255) tttCTGGTGGAGAACGGCCTGGGCGCAAAAGATGa < 1:2441073/35‑1 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAAt > 1:21929/1‑35 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:2801436/1‑71 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1098744/1‑71 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:2938143/1‑71 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1255124/1‑71 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1268576/1‑71 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:2785949/1‑71 (MQ=255) tCTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa > 1:1706385/1‑71 (MQ=255) cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:2603691/70‑1 (MQ=255) cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:647429/70‑1 (MQ=255) cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:3200956/70‑1 (MQ=255) cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:2779614/70‑1 (MQ=255) cTGGTGGAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCa < 1:2903610/70‑1 (MQ=255) tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:509851/38‑1 (MQ=255) tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:2394010/38‑1 (MQ=255) tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1777060/38‑1 (MQ=255) tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1439847/38‑1 (MQ=255) tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:1002378/38‑1 (MQ=255) tggAGAACGGCCTGGGCGCAAAAGATGAATTTGCTGcc < 1:3198972/38‑1 (MQ=255) | CGGTCTGCGTATCACCATGAATATGATGTACGATCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACGGCCTGGGCGCAAAAGATGAATTTGCTGCCAATGGCGAGATTAACGACGACTATCGCA > W3110S.gb/2840698‑2840822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |