| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,841,727 | C→T | 77.2% | A126T (GCC→ACC) | hycH ← | protein required for maturation of hydrogenase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,841,727 | 0 | C | T | 77.2% | 206.6 / 62.0 | 105 | A126T (GCC→ACC) | hycH | protein required for maturation of hydrogenase 3 |
| Reads supporting (aligned to +/- strand): ref base C (16/8); new base T (40/41); total (56/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAA > W3110S.gb/2841664‑2841791 | catGCTATTGCCAACTCAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTcc > 1:3268086/1‑71 (MQ=255)catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTcc > 1:1870702/1‑71 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:965519/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:3153439/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:570172/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:2611776/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:2282952/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:2157816/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:778045/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:1870449/71‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCt < 1:1686830/71‑1 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgatg < 1:633491/69‑1 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTcat > 1:1872680/1‑61 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAata > 1:1882932/1‑71 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAata > 1:2387421/1‑71 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAat > 1:1852053/1‑70 (MQ=255) cAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATc < 1:1067824/71‑1 (MQ=255) cAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATc < 1:943329/71‑1 (MQ=255) cAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATc < 1:580096/71‑1 (MQ=255) aaCACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCa < 1:2440487/71‑1 (MQ=255) tcaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:2878138/2‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:3555387/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:1044073/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:2966865/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:1207469/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:1859511/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:1283088/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtgat > 1:1377191/1‑60 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCtga > 1:2730809/1‑59 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtga > 1:401929/1‑59 (MQ=255) acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtga > 1:2976527/1‑59 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGgtgt < 1:212224/45‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:385292/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1474432/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:3004849/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2205157/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1007662/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2163439/71‑1 (MQ=255) agtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:2821686/1‑70 (MQ=255) gtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:463267/64‑1 (MQ=255) gtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:205154/64‑1 (MQ=255) gtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2638027/64‑1 (MQ=255) gtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1109477/64‑1 (MQ=255) gtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2132030/64‑1 (MQ=255) gtcaCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCa < 1:2049960/71‑1 (MQ=255) caCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTaa > 1:2057050/1‑64 (MQ=255) caCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTaa > 1:470079/1‑64 (MQ=255) caCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCAtt > 1:827321/1‑71 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg > 1:1426813/1‑71 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:1461637/71‑1 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:1882324/71‑1 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:714902/71‑1 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:2928745/71‑1 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:2148739/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGACTATCATGCAGCATACTTAACAGCATTGTACTc < 1:3161663/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:1915441/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:3649195/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:3307020/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:2075665/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:2433779/71‑1 (MQ=255) gTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTc < 1:3484287/71‑1 (MQ=255) ctcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCa > 1:3075439/1‑71 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTTATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:2697660/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAat < 1:3322568/37‑1 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAat < 1:1133993/37‑1 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:2750956/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:2697880/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:3256169/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:459025/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:1886864/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:620185/1‑65 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAt > 1:416088/1‑71 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAt > 1:2816484/1‑71 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAt > 1:1037186/1‑71 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAt > 1:2822004/1‑71 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAt > 1:2817838/1‑71 (MQ=255) tcctcACCATCAAATAGATGGTGTTTTCATGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:887110/1‑65 (MQ=255) ctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTc > 1:3700329/1‑71 (MQ=255) ctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTc > 1:2947241/1‑71 (MQ=255) aCCATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctg > 1:215467/1‑71 (MQ=255) ccATCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:946849/57‑1 (MQ=255) tCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACtt < 1:958810/44‑1 (MQ=255) tCAAATAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgctgc > 1:3322813/1‑71 (MQ=255) cAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCctgctgct > 1:2976567/1‑71 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTcc > 1:744924/1‑58 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCa > 1:2559992/1‑59 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCa > 1:583671/1‑59 (MQ=255) aaaTAGATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCa > 1:3586626/1‑59 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGTAGCATACTTAACAGCATTGTACTCCATTCctgctgctg > 1:3693462/1‑70 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:2464701/1‑71 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:1917040/1‑71 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:1878975/1‑71 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:845371/1‑71 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:132218/1‑71 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:420031/1‑71 (MQ=255) aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCtgctgctgc > 1:3471067/1‑71 (MQ=255) gATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGaa < 1:2313171/70‑1 (MQ=255) gATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGaa < 1:2771104/70‑1 (MQ=255) gATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGaa < 1:2929534/70‑1 (MQ=255) gATGGTGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGaa < 1:442125/70‑1 (MQ=255) | CATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAA > W3110S.gb/2841664‑2841791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |