| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,860,110 | G→A | 80.0% | V9I (GTC→ATC) | ygbJ → | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,860,110 | 0 | G | A | 80.0% | 115.8 / 26.0 | 55 | V9I (GTC→ATC) | ygbJ | predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (3/8); new base A (23/21); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCG > W3110S.gb/2860043‑2860167 | acaATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATc < 1:1270180/70‑1 (MQ=255) aaTATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGt < 1:1557021/71‑1 (MQ=255) atatCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGt < 1:2690112/70‑1 (MQ=255) atatCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGt < 1:509683/70‑1 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:2725304/1‑71 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:224161/1‑71 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:2158408/1‑71 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:2810451/1‑71 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:2855965/1‑71 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:3282643/1‑71 (MQ=255) tCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:896055/1‑71 (MQ=255) tCAAAAAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCg > 1:3235574/1‑71 (MQ=255) caaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTgg > 1:796038/1‑70 (MQ=255) caaaATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTgg > 1:1381292/1‑70 (MQ=255) aCTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCTTTGGCTTAGGGTc < 1:2781320/70‑1 (MQ=255) aCTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTc < 1:2991853/70‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatggg < 1:2619434/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatggg < 1:2614976/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCaatggg < 1:115633/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:680165/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:352546/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:1431785/71‑1 (MQ=255) aaTTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgg < 1:3085663/70‑1 (MQ=255) ggAGAGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagc < 1:1010364/71‑1 (MQ=255) gagtGCAGATGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:919678/61‑1 (MQ=255) agagCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagcag < 1:2216634/71‑1 (MQ=255) aTGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgggaatgg < 1:1855196/58‑1 (MQ=255) aTGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:3395278/53‑1 (MQ=255) aTGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:488166/53‑1 (MQ=255) aTGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:954286/53‑1 (MQ=255) aTGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatggg < 1:2005843/53‑1 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGc > 1:324936/1‑38 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaa > 1:1534013/1‑48 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaa > 1:2904784/1‑48 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaa > 1:2007552/1‑48 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaa > 1:3202827/1‑48 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaa > 1:1736357/1‑48 (MQ=255) tGAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:1964824/71‑1 (MQ=255) gAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:1978984/1‑70 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCaatgggaa > 1:1770341/1‑53 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:506085/1‑64 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAgcagca > 1:194527/1‑64 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:1783554/1‑70 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:71014/1‑70 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:3448083/1‑70 (MQ=255) gAAAACGGGATCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa > 1:1281367/1‑70 (MQ=255) aTCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCcgcg > 1:340640/1‑71 (MQ=255) aTCTGAGTTTCATATCGGTATAGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCcgcg > 1:2013048/1‑71 (MQ=255) tCTGAGTTTCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:1074575/60‑1 (MQ=255) gTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:3629885/55‑1 (MQ=255) gTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:1898197/55‑1 (MQ=255) gTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:2837502/55‑1 (MQ=255) gTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCa < 1:2524758/55‑1 (MQ=255) tttCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagcagc < 1:1847786/47‑1 (MQ=255) tttCATATCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGagcagc < 1:2110095/47‑1 (MQ=255) | ACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGGATCTGAGTTTCATGTCGGTATCGTTGGCTTAGGGTCAATGGGAATGGGAGCAGCACTGTCATATGTCCGCG > W3110S.gb/2860043‑2860167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |