| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,885,984 | C→T | 75.0% | intergenic (‑109/+250) | ygcB ← / ← cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,885,984 | 0 | C | T | 75.0% | 121.1 / 42.5 | 68 | intergenic (‑109/+250) | ygcB/cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base C (7/10); new base T (20/31); total (27/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCC > W3110S.gb/2885918‑2886051 | gggCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAaag < 1:557246/71‑1 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAaagaa > 1:1792885/1‑69 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAaagaa > 1:3038587/1‑69 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAaagaa > 1:3030359/1‑69 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAaag < 1:2623572/67‑1 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAaag < 1:1399358/67‑1 (MQ=255) tATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAaag < 1:2682088/67‑1 (MQ=255) tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga < 1:90202/69‑1 (MQ=255) tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga < 1:178427/69‑1 (MQ=255) tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga < 1:1675540/69‑1 (MQ=255) tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga < 1:1528495/69‑1 (MQ=255) tttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAagaaga < 1:3082496/69‑1 (MQ=255) ttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGAc > 1:3500831/1‑71 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAgaga > 1:288236/1‑69 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAgaga > 1:3421998/1‑69 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAgaga > 1:1143549/1‑69 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAgaga > 1:2263479/1‑69 (MQ=255) tttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGAc > 1:282455/1‑70 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt > 1:3278749/1‑70 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt > 1:3274445/1‑70 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt > 1:1435604/1‑70 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt > 1:520186/1‑70 (MQ=255) ttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt > 1:2523487/1‑70 (MQ=255) ttCGATGATGCTCATAAATATCGAATTACAGTGATATACACATGTATTGCCTCCCTGTAAAGAAGAGACt < 1:791748/70‑1 (MQ=255) gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTc > 1:963649/1‑71 (MQ=255) gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTc > 1:369568/1‑71 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGt < 1:267302/71‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTg < 1:135191/70‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTg < 1:3652317/70‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTg < 1:1562826/70‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTg < 1:2958557/70‑1 (MQ=255) aTAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTg < 1:2331466/70‑1 (MQ=255) tatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:1008363/71‑1 (MQ=255) tCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:2484186/69‑1 (MQ=255) tCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:382434/69‑1 (MQ=255) tCGAATTACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTggg < 1:421623/69‑1 (MQ=255) ttACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt < 1:186835/45‑1 (MQ=255) ttACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt < 1:2449449/45‑1 (MQ=255) ttACAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACt < 1:3454372/45‑1 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTaa > 1:1356269/1‑45 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTaa > 1:1417332/1‑45 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTaa > 1:3127122/1‑45 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACg < 1:3639024/70‑1 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACg < 1:931901/70‑1 (MQ=255) aCAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGc > 1:2475800/1‑71 (MQ=255) gATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTg < 1:2886130/51‑1 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCg < 1:3382354/57‑1 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCg < 1:1192199/57‑1 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCg < 1:2222446/57‑1 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCg < 1:2314480/57‑1 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCg < 1:1712088/57‑1 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg > 1:1986743/1‑71 (MQ=255) taCACATGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAg > 1:2200779/1‑71 (MQ=255) cacaTGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTg > 1:551332/1‑45 (MQ=255) tGTCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGa < 1:2048215/52‑1 (MQ=255) tGTCCTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGa < 1:3576247/52‑1 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGaa < 1:2557891/36‑1 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGaa < 1:2263004/36‑1 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGaa < 1:1941030/36‑1 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 1:3264576/1‑68 (MQ=255) tCTTGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGa > 1:1926121/1‑68 (MQ=255) ttGCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa > 1:426919/1‑70 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGaaa > 1:3065051/1‑70 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:288769/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:1715416/68‑1 (MQ=255) gCCTCCCTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGa < 1:59024/68‑1 (MQ=255) gCCTCCCTACAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGaaa < 1:628792/70‑1 (MQ=255) cTGTAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGcc < 1:2256509/71‑1 (MQ=255) | GGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCC > W3110S.gb/2885918‑2886051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |