| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,910,539 | C→A | 100% | R590L (CGC→CTC) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,910,539 | 0 | C | A | 93.6% | 118.5 / 3.9 | 47 | R590L (CGC→CTC) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base A (20/24); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTC > W3110S.gb/2910481‑2910600 | gATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggcg < 1:599054/71‑1 (MQ=255) gtgCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTg > 1:106490/1‑60 (MQ=255) gtgCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTg > 1:1763460/1‑60 (MQ=255) gtgCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGc > 1:3080793/1‑71 (MQ=255) cATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:2903693/70‑1 (MQ=255) cATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:1815373/70‑1 (MQ=255) cATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:3566331/70‑1 (MQ=255) cATCAGGTAGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:236433/70‑1 (MQ=255) aGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtttttt > 1:1981890/1‑70 (MQ=255) gCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:2928402/70‑1 (MQ=255) gCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:370793/70‑1 (MQ=255) gCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:649088/70‑1 (MQ=255) gCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:2056868/70‑1 (MQ=255) cacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGtt < 1:2192378/69‑1 (MQ=255) cacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGtt < 1:1019518/69‑1 (MQ=255) cacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGtt < 1:2161138/69‑1 (MQ=255) cacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGtt < 1:1948668/69‑1 (MQ=255) ttCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTgcggc > 1:1147492/1‑43 (MQ=255) ttCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCttttt > 1:1088335/1‑55 (MQ=255) ttCGACTACCACGCGACCGTTATCTTTACTGAGGttt > 1:1153927/1‑37 (MQ=255) ttCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtttt > 1:2475490/1‑54 (MQ=255) tCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAg > 1:194778/1‑71 (MQ=255) tCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAg > 1:1901659/1‑71 (MQ=255) cGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAgc > 1:1158624/1‑71 (MQ=255) gACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCtt < 1:324462/49‑1 (MQ=255) tACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCgcgg > 1:3064513/1‑71 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCgtgt > 1:687327/1‑40 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCttttt > 1:1915483/1‑48 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCttttt > 1:495620/1‑48 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCttttt > 1:1294257/1‑48 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCttttt > 1:1651126/1‑48 (MQ=255) aCCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGc > 1:1308632/1‑60 (MQ=255) ccACGCGACCGTTATCTTTACTGAGGTTTTGCGGCgtg > 1:82774/1‑38 (MQ=255) ccACGCGACCGTTATCTTTACTGAGGTTTTGCGGCgtg > 1:539053/1‑38 (MQ=255) ccACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCgcggcg > 1:234141/1‑71 (MQ=255) ccACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTAAGCgcggcg > 1:2796062/1‑71 (MQ=255) cGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGc < 1:2690655/71‑1 (MQ=255) cGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGc < 1:3280833/71‑1 (MQ=255) cGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGc < 1:354720/71‑1 (MQ=255) cGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGc < 1:1871580/71‑1 (MQ=255) cGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGc < 1:1900440/71‑1 (MQ=255) aTCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCtt > 1:1989024/1‑71 (MQ=255) tCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:1229931/39‑1 (MQ=255) tCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:2297484/39‑1 (MQ=255) tCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGa < 1:1854102/39‑1 (MQ=255) tACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTc < 1:737202/67‑1 (MQ=255) tACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTc < 1:2797933/67‑1 (MQ=255) | GATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTC > W3110S.gb/2910481‑2910600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |