| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,916,386 | G→A | 100% | E892K (GAA→AAA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,916,386 | 0 | G | A | 86.0% | 127.0 / 13.4 | 50 | E892K (GAA→AAA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (2/5); new base A (17/26); total (19/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
TATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAA > W3110S.gb/2916317‑2916448 | tataGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaag > 1:3593709/1‑70 (MQ=255) taGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaaga < 1:1976922/69‑1 (MQ=255) cGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag < 1:2950293/71‑1 (MQ=255) cGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag < 1:2800270/71‑1 (MQ=255) cGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag < 1:1628049/71‑1 (MQ=255) cGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag < 1:2252531/71‑1 (MQ=255) gttgtgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAaaaagaaga < 1:635421/69‑1 (MQ=255) ggTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAaaaagaaga < 1:1977642/71‑1 (MQ=255) ggTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAaaaagaaga < 1:426361/71‑1 (MQ=255) tgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGAttt < 1:1768882/70‑1 (MQ=255) tgCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTg > 1:280237/1‑71 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg > 1:332866/1‑70 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg > 1:2246787/1‑70 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg < 1:1133086/70‑1 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg > 1:2707357/1‑70 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg < 1:2906109/70‑1 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg > 1:2993852/1‑70 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg < 1:1396234/70‑1 (MQ=255) ccACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTgg < 1:1793764/70‑1 (MQ=255) tGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAg < 1:1789543/71‑1 (MQ=255) tGAAGAAACTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAaaaagaaga > 1:2552268/1‑58 (MQ=39) aagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAgct > 1:3073101/1‑71 (MQ=255) aagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAgct > 1:212342/1‑71 (MQ=255) aagaaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAgct > 1:1740479/1‑71 (MQ=255) aaTCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAgctgct > 1:1769067/1‑71 (MQ=255) tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGctgctg > 1:2599149/1‑70 (MQ=255) tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGctgctg > 1:2602485/1‑70 (MQ=255) tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGctgctg > 1:202591/1‑70 (MQ=255) tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGctgctg > 1:1634847/1‑70 (MQ=255) tctcTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGctgctg > 1:2468795/1‑70 (MQ=255) tGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCgg < 1:2611591/58‑1 (MQ=255) tGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCgg < 1:3845/58‑1 (MQ=255) aaCTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTg < 1:785777/71‑1 (MQ=255) cTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAg < 1:397140/54‑1 (MQ=255) cTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAg < 1:2119282/54‑1 (MQ=255) tGATCGAACACCAGCTACGTAGCGGTACTAaaaaagaag > 1:2658049/1‑39 (MQ=37) tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTggtc > 1:684180/1‑69 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag > 1:1596510/1‑38 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag > 1:141662/1‑38 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag > 1:504041/1‑38 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag > 1:299087/1‑38 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAaaaaagaag > 1:3318914/1‑38 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTgg > 1:3239149/1‑68 (MQ=255) gATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGa > 1:3134337/1‑69 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:2591566/71‑1 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:93173/71‑1 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:1762350/71‑1 (MQ=255) aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:2432163/71‑1 (MQ=255) tCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:3000763/70‑1 (MQ=255) acaacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATgg < 1:2437029/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:1290522/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:772394/71‑1 (MQ=255) aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:2697092/71‑1 (MQ=255) aacaGCTACGTAGCGGTAATAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGTAt < 1:919062/71‑1 (MQ=255) acaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt < 1:2627541/70‑1 (MQ=255) aCGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcg > 1:3465082/1‑71 (MQ=255) gCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTcgcgcgg > 1:3278080/1‑70 (MQ=255) gTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGaa < 1:2490724/71‑1 (MQ=255) tACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:343388/48‑1 (MQ=255) tACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:860294/48‑1 (MQ=255) cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag < 1:3187656/47‑1 (MQ=255) cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag < 1:1730165/47‑1 (MQ=255) cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACgag < 1:1565210/47‑1 (MQ=255) tAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:416979/45‑1 (MQ=255) tAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:1725175/45‑1 (MQ=255) tAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga < 1:496796/45‑1 (MQ=255) aaaaaaGAAGATTTGGAACCGGAGCTGCTGGAACTg < 1:3109294/36‑1 (MQ=255) | TATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAA > W3110S.gb/2916317‑2916448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |