| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,931,037 | C→A | 71.5% | A212A (GCG→GCT) | fucO ← | L‑1,2‑propanediol oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,931,037 | 0 | C | A | 71.5% | 38.9 / 47.0 | 51 | A212A (GCG→GCT) | fucO | L‑1,2‑propanediol oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (5/9); new base A (23/13); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
CCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTT > W3110S.gb/2930981‑2931104 | ccTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACg > 1:3310600/1‑71 (MQ=255)ccTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACg > 1:1252615/1‑71 (MQ=255)ccTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACg > 1:2222252/1‑71 (MQ=255) cTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTA‑‑GCCCACGCGCCACggg < 1:1375370/70‑1 (MQ=255) gCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACggg < 1:3225218/69‑1 (MQ=255) gCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACggg < 1:760848/69‑1 (MQ=255) cAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:577880/71‑1 (MQ=255) cAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:3300873/71‑1 (MQ=255) aGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:15897/70‑1 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata < 1:313933/71‑1 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata < 1:2388801/71‑1 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata < 1:2899788/71‑1 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata < 1:1227382/71‑1 (MQ=255) gcgcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata < 1:2658519/71‑1 (MQ=255) gcCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAAtata < 1:3420735/71‑1 (MQ=255) ccccAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAc < 1:898041/71‑1 (MQ=255) ccccAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAc < 1:1238623/71‑1 (MQ=255) ccAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAccc < 1:3648757/71‑1 (MQ=255) aTGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAcccc > 1:1108046/1‑66 (MQ=255) aTGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAcccc > 1:3374218/1‑66 (MQ=255) aTGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAcccc > 1:407022/1‑66 (MQ=255) aTGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATAcccc > 1:952538/1‑66 (MQ=255) aTGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCAAGCGCCACGGGTAATATAcc > 1:3439360/1‑64 (MQ=255) aTGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCAACGCGCCACGGGTAATATAcccc > 1:2366300/1‑66 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:3107071/57‑1 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTaa < 1:2049225/57‑1 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata > 1:296791/1‑59 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata > 1:3293544/1‑59 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata > 1:3378020/1‑59 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata > 1:2406600/1‑59 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata > 1:345785/1‑59 (MQ=255) tGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAata > 1:471855/1‑59 (MQ=255) tCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCCCGCGCCAAGGGTAATATACCCCTCAATAGCAt > 1:491063/1‑70 (MQ=255) tCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCAt > 1:2439670/1‑70 (MQ=255) tCAATCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCAt > 1:3722688/1‑70 (MQ=255) aTCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCAtgagt < 1:1453550/71‑1 (MQ=255) aTCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCAtgagt < 1:2732324/71‑1 (MQ=255) aTCGCTTTAATGTGCAGTGCATCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCAtgagt < 1:446466/71‑1 (MQ=255) tttAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACTGGTaa < 1:974880/44‑1 (MQ=255) tttAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTaa < 1:988536/44‑1 (MQ=255) tAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata > 1:3684598/1‑44 (MQ=255) tAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata > 1:1484203/1‑44 (MQ=255) tAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAata > 1:3242593/1‑44 (MQ=255) tgcagtgcaTCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGa < 1:2882336/70‑1 (MQ=255) caTCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTc > 1:2779643/1‑71 (MQ=255) caTCGGTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTc > 1:889633/1‑71 (MQ=255) ggTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAg > 1:237907/1‑41 (MQ=255) ggTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAgcg > 1:2825925/1‑53 (MQ=255) ggTTAGAGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAgcg > 1:1026012/1‑53 (MQ=255) tAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCtt > 1:2399814/1‑71 (MQ=255) tAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCtt > 1:2268418/1‑71 (MQ=255) | CCTCGCAGCGCCCCAGCAATGATTTCAATCGCTTTAATGTGCAGTGCATCGGTTAGCGCCCACGCGCCACGGGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTT > W3110S.gb/2930981‑2931104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |