| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,938,519 | A→G | 70.4% | I166V (ATC→GTC) | fucR → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,938,519 | 0 | A | G | 70.4% | 80.4 / 41.4 | 54 | I166V (ATC→GTC) | fucR | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base A (13/3); new base G (18/20); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCACACTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCC > W3110S.gb/2938451‑2938578 | gcACACTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTc < 1:3187052/71‑1 (MQ=255) cacaCTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCg < 1:695226/71‑1 (MQ=255) cacTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGAt > 1:1284888/1‑71 (MQ=255) gcgcAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGttt < 1:1608387/70‑1 (MQ=255) gcAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTAtt < 1:1144367/71‑1 (MQ=255) gcAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTAtt < 1:1439698/71‑1 (MQ=255) gcAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTAtt < 1:1315228/71‑1 (MQ=255) tatGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTc > 1:1674791/1‑71 (MQ=255) atGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCt > 1:2769876/1‑71 (MQ=255) cTGTTACGGCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:842903/71‑1 (MQ=255) ttACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:766160/68‑1 (MQ=255) ttACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTtgtg < 1:2395520/68‑1 (MQ=255) ttACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGa < 1:122633/69‑1 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATc > 1:3513302/1‑49 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATc > 1:1133335/1‑49 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATc > 1:2141472/1‑49 (MQ=255) tACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATc > 1:1842498/1‑49 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGAt > 1:2315874/1‑47 (MQ=255) aCGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGAt > 1:2711303/1‑47 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:1239033/71‑1 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:3040324/71‑1 (MQ=255) tCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATc < 1:2685311/71‑1 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:2003488/1‑63 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:1612104/1‑63 (MQ=255) aaTCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTAGTGaa > 1:3666674/1‑63 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:1902696/1‑59 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGaa > 1:2808671/1‑59 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:3567514/1‑70 (MQ=255) ccTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATag > 1:282391/1‑70 (MQ=255) cTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATa > 1:698071/1‑68 (MQ=255) cTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATa > 1:3417023/1‑68 (MQ=255) cTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATa > 1:2345708/1‑68 (MQ=255) cTCGCTGATTTCCCAACTTAAATCGCTCGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATa > 1:2738465/1‑68 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGAt > 1:770969/1‑60 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGAt > 1:2264802/1‑60 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGAt > 1:1346331/1‑60 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGAt > 1:1784434/1‑60 (MQ=255) gCTGATTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGCGAt > 1:1115801/1‑60 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:3575806/70‑1 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAgcggcg < 1:57621/70‑1 (MQ=255) aTTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGgcgc < 1:1280946/71‑1 (MQ=255) ttCGCAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCa < 1:3306333/70‑1 (MQ=255) tCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCAc < 1:3350229/70‑1 (MQ=255) tCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCAc < 1:21829/70‑1 (MQ=255) cccAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACtg > 1:714267/1‑71 (MQ=255) ccAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACtgt < 1:833323/71‑1 (MQ=255) ccAACTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACtgt < 1:3051751/71‑1 (MQ=255) aaCTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTgg > 1:1785328/1‑71 (MQ=255) aaCTTAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTgg > 1:1714568/1‑71 (MQ=255) tAAATCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATa > 1:1524719/1‑50 (MQ=255) tAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGaa > 1:1190856/1‑40 (MQ=255) tAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGaa > 1:859906/1‑40 (MQ=255) aaaTCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACt < 1:723540/70‑1 (MQ=255) aaTCGCTGGAAGTCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTcc < 1:1864026/71‑1 (MQ=255) | GCACACTTGAGCGCAAATATGGCTGTTACGTCAATCCCTCGCTGATTTCCCAACTTAAATCGCTGGAAATCGATCTGTTTATTTTTTCTTGTGAAGGGATCGATAGCAGCGGCGCACTGTGGGACTCC > W3110S.gb/2938451‑2938578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |