| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,002,965 | A→G | 8.3% | L493S (TTA→TCA) | ygeV ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,002,965 | 0 | A | G | 8.3% | 201.2 / 7.9 | 72 | L493S (TTA→TCA) | ygeV | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (35/31); new base G (4/2); total (39/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAT > W3110S.gb/3002902‑3003034 | aGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgcg > 1:737552/1‑71 (MQ=255)aGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgcg > 1:1236077/1‑71 (MQ=255)aGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgcg > 1:1360445/1‑71 (MQ=255)aGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgcg > 1:527323/1‑71 (MQ=255) gCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCATATATTCCATCAAATTGCTTGACTcgcg > 1:1031932/1‑70 (MQ=255) gCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTGACTcgcg > 1:598483/1‑70 (MQ=255) gCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTGACTcgcg > 1:2971892/1‑70 (MQ=255) gCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTGACTcgcg > 1:2063688/1‑70 (MQ=255) tCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTcc > 1:2202958/1‑71 (MQ=255) gATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTcgc > 1:1743575/1‑59 (MQ=255) gATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCgg > 1:2451274/1‑71 (MQ=255) gATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCgg > 1:3531746/1‑71 (MQ=255) aCTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGcc < 1:502777/70‑1 (MQ=255) aCTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGcc < 1:3568640/70‑1 (MQ=255) ttCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGttt < 1:499882/61‑1 (MQ=255) ttCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGttt < 1:2620885/61‑1 (MQ=255) ttCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGttt < 1:2060168/61‑1 (MQ=255) ttCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGttt < 1:1691564/61‑1 (MQ=255) ttCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGttt < 1:619773/61‑1 (MQ=255) tCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc > 1:1301223/1‑70 (MQ=255) tCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc > 1:529908/1‑70 (MQ=255) cACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc < 1:702915/69‑1 (MQ=255) cACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGa > 1:3533026/1‑71 (MQ=255) cACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGa > 1:2324337/1‑71 (MQ=255) tGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAAc < 1:68415/65‑1 (MQ=255) aaGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTa < 1:775162/71‑1 (MQ=255) aaGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTa < 1:1259215/71‑1 (MQ=255) aGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTa < 1:2734836/70‑1 (MQ=255) aGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGAAg > 1:792980/1‑48 (MQ=255) aaCCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCa < 1:1608875/71‑1 (MQ=255) aCCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCa < 1:1313996/70‑1 (MQ=255) cACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAata > 1:2789712/1‑71 (MQ=255) cACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAata > 1:268020/1‑71 (MQ=255) aCGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGa > 1:672738/1‑57 (MQ=255) aCGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATg < 1:457337/59‑1 (MQ=255) aCGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATg < 1:3247418/59‑1 (MQ=255) aCGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATg < 1:2109002/59‑1 (MQ=255) ttAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTc > 1:1308977/1‑71 (MQ=255) ttAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTc > 1:1630281/1‑71 (MQ=255) aCCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgac < 1:3134844/71‑1 (MQ=255) aCCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcgac < 1:573961/71‑1 (MQ=255) aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCgacgac < 1:149482/71‑1 (MQ=255) aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCgacgac < 1:1563488/71‑1 (MQ=255) gATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGTAATATTTCGACGACa < 1:565912/71‑1 (MQ=255) gATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACa < 1:1922896/71‑1 (MQ=255) gATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACa < 1:2799418/71‑1 (MQ=255) tatTCCATCAAATTGCTTGACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACAt < 1:106943/70‑1 (MQ=255) tatTCCATCAAATTGCTTGACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACAt < 1:266729/70‑1 (MQ=255) tatTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATc < 1:1219676/71‑1 (MQ=255) tatTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATc < 1:2589583/71‑1 (MQ=255) tatTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATc < 1:1331804/71‑1 (MQ=255) atTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCa < 1:306937/54‑1 (MQ=255) atTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCa < 1:2909136/54‑1 (MQ=255) atTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCg > 1:321150/1‑71 (MQ=255) atTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCg > 1:1381822/1‑71 (MQ=255) ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGa > 1:464936/1‑41 (MQ=255) ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGa > 1:153737/1‑41 (MQ=255) ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCgg > 1:1165785/1‑69 (MQ=255) ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCgg > 1:1308204/1‑69 (MQ=255) cAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCaa > 1:3077342/1‑71 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAata > 1:3473489/1‑49 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAata > 1:895717/1‑49 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAata > 1:441822/1‑49 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcga > 1:1626820/1‑55 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcga > 1:3663947/1‑55 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTcga > 1:112957/1‑55 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAAt > 1:586235/1‑71 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAAt > 1:594340/1‑71 (MQ=255) aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAAt < 1:35951/71‑1 (MQ=255) aaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCaa < 1:1645306/46‑1 (MQ=255) aTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACa > 1:553718/1‑58 (MQ=255) tGCTTAACTCGCGCAGGTTTCAGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGc > 1:1675368/1‑69 (MQ=255) aaCTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAt > 1:1401253/1‑70 (MQ=255) aaCTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAt > 1:222711/1‑70 (MQ=255) aaCTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAt > 1:2504312/1‑70 (MQ=255) | AGCGTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGAT > W3110S.gb/3002902‑3003034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |