| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,013,681 | G→A | 79.7% | V11V (GTC→GTT) | yqeC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,013,681 | 0 | G | A | 79.7% | 133.4 / 31.9 | 64 | V11V (GTC→GTT) | yqeC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/10); new base A (24/27); total (27/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
AGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAA > W3110S.gb/3013614‑3013740 | agcTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACt < 1:1670619/71‑1 (MQ=255)agcTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:3347702/71‑1 (MQ=255) ttttCCCCCCAGCACCAACAACAGAAATCACATTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc < 1:3701193/71‑1 (MQ=255) ttttCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc < 1:3445921/71‑1 (MQ=255) tttCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGcc > 1:1001011/1‑70 (MQ=255) ttCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGa > 1:3149557/1‑71 (MQ=255) cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAAc > 1:3068398/1‑70 (MQ=255) cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAAc > 1:1077334/1‑70 (MQ=255) cACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGACGATGGGTCAAc > 1:1513562/1‑70 (MQ=255) aCCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACta > 1:3493749/1‑71 (MQ=255) aCCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACta > 1:908580/1‑71 (MQ=255) caacaaCAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACtata > 1:1485062/1‑71 (MQ=255) caacaaCAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACtata > 1:2818657/1‑71 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:1264673/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:1377825/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:1387277/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:694116/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:2585821/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:253503/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACt < 1:3673428/38‑1 (MQ=255) tCACAGTGGGACGTTTCTGCGCACCT‑AATCAATAACt < 1:836670/37‑1 (MQ=37) tCACAGTGGGACGTTGCTGCGCACCTAAATCATTAACt < 1:2542851/38‑1 (MQ=25) caGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:93728/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:2602599/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:3685299/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:483089/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:2933118/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:3307830/71‑1 (MQ=255) aGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:1445760/70‑1 (MQ=255) ggACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATg < 1:3239709/70‑1 (MQ=255) ggACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATg < 1:2002977/70‑1 (MQ=255) tttCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:2175643/61‑1 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACtata > 1:456449/1‑44 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:3223793/1‑61 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:416349/1‑61 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct > 1:264060/1‑71 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct > 1:3514132/1‑71 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct > 1:3002013/1‑71 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct > 1:2007568/1‑71 (MQ=255) tCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct > 1:2427441/1‑71 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:997007/41‑1 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:447731/41‑1 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:623137/41‑1 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:76967/41‑1 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:3268935/41‑1 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:1154377/41‑1 (MQ=255) ccTAAATCAATAACTAATGCCGATGGGTCAACTATACtttt < 1:2192375/41‑1 (MQ=255) tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:1235992/1‑51 (MQ=255) tAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:1240573/1‑51 (MQ=255) aaTCAATGACTAATGCCGATGGGTCAACTATACtttt < 1:2444297/37‑1 (MQ=255) aaTCAATGACTAATGCCGATGGGTCAACTATACtttt < 1:1417541/37‑1 (MQ=255) aaTCAATGACTAATGCCGATGGGTCAACTATACtttt < 1:2156882/37‑1 (MQ=255) aaTCAATGACTAATGCCGATGGGTCAACTATACtttt < 1:1928753/37‑1 (MQ=255) aaTCAATGACTAATGCCGATGGGTCAACTATACtttt < 1:1776853/37‑1 (MQ=255) aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:1812760/47‑1 (MQ=255) aaTCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:2321220/47‑1 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACg > 1:3393307/1‑39 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:3696834/1‑47 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:673556/1‑47 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:1413737/1‑47 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:1198519/1‑47 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:3254358/1‑47 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTa > 1:1016584/1‑47 (MQ=255) tCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATaaa > 1:3082473/1‑65 (MQ=255) | AGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAA > W3110S.gb/3013614‑3013740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |