| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,061,031 | A→G | 100% | Q509R (CAG→CGG) | yliK → | methylmalonyl‑CoA mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,061,031 | 0 | A | G | 96.8% | 100.4 / ‑3.9 | 31 | Q509R (CAG→CGG) | yliK | methylmalonyl‑CoA mutase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/15); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTG > W3110S.gb/3060970‑3061089 | cgcgCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACg > 1:1028100/1‑70 (MQ=255)cgcgCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACg > 1:105130/1‑70 (MQ=255)cgcgCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACg > 1:2001534/1‑70 (MQ=255)cgcgCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACg > 1:1480049/1‑70 (MQ=255)cgcgCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACg > 1:1769349/1‑70 (MQ=255)cgcgCAACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACg > 1:1159437/1‑70 (MQ=255) gcgcCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGaa < 1:3640702/71‑1 (MQ=255) gcgcCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGaa < 1:111613/71‑1 (MQ=255) gcgcCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGaa < 1:1813852/71‑1 (MQ=255) ccGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACctgc > 1:2831494/1‑71 (MQ=255) cGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACctgct > 1:3710696/1‑71 (MQ=255) cGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACctgct > 1:2406711/1‑71 (MQ=255) ccgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGtt < 1:1725788/71‑1 (MQ=255) ccgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGtt < 1:163409/71‑1 (MQ=255) ccgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGtt < 1:1352172/71‑1 (MQ=255) cgTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGtt < 1:3298701/70‑1 (MQ=255) aCCGCCGCGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGc > 1:2165759/1‑71 (MQ=255) gccgcGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAAcc > 1:3265017/1‑46 (MQ=255) gccgcGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCg > 1:2635517/1‑70 (MQ=255) gccgcGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGc > 1:1577298/1‑71 (MQ=255) gccgcGTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGc > 1:3221374/1‑71 (MQ=255) gcgTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTcg < 1:1102486/71‑1 (MQ=255) gcgTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTcg < 1:123410/71‑1 (MQ=255) gcgTTGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTcg < 1:3260278/71‑1 (MQ=255) ttGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGt < 1:1035469/71‑1 (MQ=255) ttGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGt < 1:3587416/71‑1 (MQ=255) ttGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGt < 1:26397/71‑1 (MQ=255) ttGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGt < 1:2368389/71‑1 (MQ=255) ttGAACGCCCTGACTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGt < 1:2089562/71‑1 (MQ=255) cTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTg > 1:2643855/1‑71 (MQ=255) cTCACGCCGCACGGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTg > 1:161709/1‑71 (MQ=255) | CGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTG > W3110S.gb/3060970‑3061089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |