| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,135,166 | A→G | 81.1% | intergenic (‑139/+153) | pitB ← / ← gss | phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,135,166 | 0 | A | G | 81.1% | 172.9 / 32.4 | 74 | intergenic (‑139/+153) | pitB/gss | phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (12/2); new base G (26/34); total (38/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
TGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAA > W3110S.gb/3135097‑3135232 | tGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaaa > 1:3388808/1‑71 (MQ=255)tGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaaa > 1:3107375/1‑71 (MQ=255)tGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaaa > 1:2533299/1‑71 (MQ=255)tGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaaa > 1:2038479/1‑71 (MQ=255)tGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaaa > 1:1353385/1‑71 (MQ=255)tGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaaa > 1:559650/1‑71 (MQ=255) atatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt < 1:974848/71‑1 (MQ=255) atatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt < 1:2287291/71‑1 (MQ=255) atatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt < 1:617122/71‑1 (MQ=255) atatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCtt > 1:3658994/1‑71 (MQ=255) atatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCtt > 1:3052351/1‑71 (MQ=255) atatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCtt > 1:2047232/1‑71 (MQ=255) tatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt > 1:2944938/1‑70 (MQ=255) tatCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt > 1:1268797/1‑70 (MQ=255) atCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt > 1:441988/1‑69 (MQ=255) tCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCtt > 1:1849102/1‑68 (MQ=255) aTTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGAc > 1:2498162/1‑63 (MQ=255) aTTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAg > 1:1733209/1‑69 (MQ=255) aTTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGa > 1:3590784/1‑70 (MQ=255) ttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAaat > 1:3452635/1‑70 (MQ=255) ttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAaat > 1:3027469/1‑70 (MQ=255) aCGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:3218757/71‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaaaa < 1:2767897/71‑1 (MQ=255) aaaaGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAacac < 1:1606950/70‑1 (MQ=255) gtgtTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTAtt > 1:1805605/1‑71 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:485951/56‑1 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:3092424/56‑1 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:2463652/56‑1 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTAt < 1:840972/67‑1 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTAt < 1:680740/67‑1 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTAt < 1:387652/67‑1 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAg > 1:1509269/1‑61 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCAc > 1:3435503/1‑70 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCAc > 1:1437795/1‑70 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:666877/1‑71 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat < 1:2417797/47‑1 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat < 1:2701830/47‑1 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat < 1:1635025/47‑1 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat < 1:532058/47‑1 (MQ=255) tCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaaaa > 1:1302461/1‑53 (MQ=255) tCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGt > 1:2335058/1‑71 (MQ=255) tCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGt > 1:963548/1‑71 (MQ=255) tCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTACACTGGt > 1:2895741/1‑71 (MQ=255) cTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTa > 1:407027/1‑39 (MQ=255) tgtATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAata < 1:1403289/71‑1 (MQ=255) aTCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:390327/43‑1 (MQ=255) tCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatatat > 1:1504691/1‑70 (MQ=255) tCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatatat > 1:1380340/1‑70 (MQ=255) tCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATAtt > 1:3393629/1‑71 (MQ=255) aaTAAGCATTCATTCAAGACCTTTAGAAATACTCAAAACACAGGTATTCCACTGGtt < 1:2560959/57‑1 (MQ=255) aaTAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGtt < 1:289825/57‑1 (MQ=255) aaTAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGtt < 1:2501434/57‑1 (MQ=255) aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACata > 1:1689813/1‑71 (MQ=255) aTAAGCATTCATTCAAAACCTTTAGAAATAATCaaaa < 1:3107001/37‑1 (MQ=255) aTAAGCATTCATTCAAAACCTTTAGAAATAATCaaaa < 1:1311014/37‑1 (MQ=255) aaGCATTCATTCAAGACCTTTAGAAATAATCAAAac > 1:1133270/1‑36 (MQ=255) aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAg > 1:1413654/1‑39 (MQ=255) aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAg > 1:1548917/1‑39 (MQ=255) aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAg > 1:2273739/1‑39 (MQ=255) aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatga < 1:1360770/70‑1 (MQ=255) aGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatga < 1:1665002/70‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTc < 1:2554542/41‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTc < 1:2751774/41‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTc < 1:1523989/41‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTc < 1:1380534/41‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:1256132/51‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:515144/51‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:3121643/51‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcaca < 1:2629080/61‑1 (MQ=255) ttcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcaca < 1:2423564/61‑1 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTa > 1:2712223/1‑36 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTa > 1:1483599/1‑36 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatgaa > 1:908897/1‑70 (MQ=255) ttcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatga < 1:3559109/62‑1 (MQ=255) ttcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatga < 1:3403410/62‑1 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACata > 1:1027444/1‑57 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACata > 1:3376397/1‑57 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa > 1:770544/1‑70 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa > 1:74525/1‑70 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa > 1:3648608/1‑70 (MQ=255) | TGAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAA > W3110S.gb/3135097‑3135232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |