| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,164,775 | C→T | 63.2% | A534T (GCA→ACA) | ygiS ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,164,775 | 0 | C | T | 63.2% | 46.4 / 60.0 | 57 | A534T (GCA→ACA) | ygiS | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (8/13); new base T (21/15); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGG > W3110S.gb/3164707‑3164843 | gAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCt > 1:50708/1‑71 (MQ=255)gAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCt > 1:269150/1‑71 (MQ=255) gTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTg < 1:2992823/71‑1 (MQ=255) acaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGata > 1:2565687/1‑71 (MQ=255) ggAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGAtata > 1:1085939/1‑70 (MQ=255) ggAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGAtata > 1:868002/1‑70 (MQ=255) ggAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGAtata > 1:1833404/1‑70 (MQ=255) cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGata > 1:997464/1‑62 (MQ=255) cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAActct > 1:2342623/1‑71 (MQ=255) cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAActct > 1:2672024/1‑71 (MQ=255) cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAActct > 1:2476120/1‑71 (MQ=255) cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAActct > 1:2016528/1‑71 (MQ=255) aaCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCtt > 1:1661633/1‑71 (MQ=255) aaCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCtt > 1:603904/1‑71 (MQ=255) aaTAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTg > 1:1629005/1‑70 (MQ=255) gcgcCGCCGGACGTGCTCTGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTa > 1:594048/1‑68 (MQ=255) gcgcCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTa > 1:1540557/1‑68 (MQ=255) gcgcCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGa < 1:2247740/70‑1 (MQ=255) cgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:417538/71‑1 (MQ=255) cgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:432149/71‑1 (MQ=255) ggACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:1600827/70‑1 (MQ=255) gTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:1560265/66‑1 (MQ=255) tGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:32097/65‑1 (MQ=255) tGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:2382981/65‑1 (MQ=255) cTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAt < 1:1283400/70‑1 (MQ=255) cTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAt < 1:1367154/70‑1 (MQ=255) cTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAt < 1:109224/70‑1 (MQ=255) ccGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTAt > 1:455126/1‑71 (MQ=255) cGACGGCGATAAAGGCATCAATCTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATg < 1:2535040/71‑1 (MQ=255) aTAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaa < 1:2949479/71‑1 (MQ=255) aTAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaa < 1:2945259/71‑1 (MQ=255) tAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:1033919/51‑1 (MQ=255) tAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa < 1:661040/71‑1 (MQ=255) tAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa < 1:1630597/71‑1 (MQ=255) gCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:528632/46‑1 (MQ=255) tCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATaa < 1:1363151/40‑1 (MQ=255) tCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATaa < 1:193028/40‑1 (MQ=255) tCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATaa < 1:1605098/40‑1 (MQ=255) tCAATGTGCCTTGATATACAACTCTTTGCTGT‑GACATaa < 1:2652033/39‑1 (MQ=39) cAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTTGGGATTATGCAGCGGAAAACCGCCAAc > 1:71419/1‑71 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATc > 1:153650/1‑40 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATc > 1:2813673/1‑40 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:3313290/1‑69 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACg > 1:2711871/1‑71 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACg > 1:1940043/1‑71 (MQ=255) tgtgTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa < 1:1385741/71‑1 (MQ=255) tgtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGa < 1:3097514/45‑1 (MQ=255) tgtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGa < 1:1382024/45‑1 (MQ=255) tgtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGa < 1:1821061/45‑1 (MQ=255) tgtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGa < 1:1753986/45‑1 (MQ=255) tgtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGa < 1:1748091/45‑1 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:341274/1‑45 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:3452445/1‑45 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:35408/1‑45 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:2982395/1‑45 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAgg > 1:3278657/1‑71 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAgg > 1:529599/1‑71 (MQ=255) | GAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGG > W3110S.gb/3164707‑3164843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |