Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 85,966 T→C 61.9% F113L (TTT→CTT)  ilvI → acetolactate synthase III, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb85,9660TC61.9% 33.9 / 43.3 42F113L (TTT→CTT) ilvIacetolactate synthase III, large subunit
Reads supporting (aligned to +/- strand):  ref base T (7/9);  new base C (8/18);  total (15/27)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

GATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGT  >  W3110S.gb/85903‑86024
                                                               |                                                          
gATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAgg                                                      <  1:829256/70‑1 (MQ=255)
 attccattccATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAg                                                       <  1:173637/68‑1 (MQ=255)
 attccattccATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAg                                                       <  1:601669/68‑1 (MQ=255)
 attccattccATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAg                                                       <  1:2334504/68‑1 (MQ=255)
 attccattccATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAg                                                       <  1:2664709/68‑1 (MQ=255)
 attccattccATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAg                                                       <  1:2794458/68‑1 (MQ=255)
      attccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGa                                               <  1:2822932/71‑1 (MQ=255)
      attccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGa                                               <  1:1688235/71‑1 (MQ=255)
      attccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGa                                               <  1:1870672/71‑1 (MQ=255)
      attccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGa                                               <  1:1767759/71‑1 (MQ=255)
      attccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGa                                               <  1:1995337/71‑1 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAt                                            >  1:30570/1‑71 (MQ=255)
         ccattAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAt                                            >  1:752451/1‑71 (MQ=255)
         ccatgAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAt                                            >  1:3553940/1‑71 (MQ=255)
            ttAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAt                                            <  1:996748/68‑1 (MQ=255)
            ttAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAt                                            <  1:2802115/68‑1 (MQ=255)
            ttAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAt                                            <  1:3650330/68‑1 (MQ=255)
            ttAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAt                                            <  1:686317/68‑1 (MQ=255)
            ttAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAt                                            <  1:767930/68‑1 (MQ=255)
              aGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAtgg                                          >  1:2963460/1‑68 (MQ=255)
              aGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAtgg                                          >  1:2967827/1‑68 (MQ=255)
                ttGTCCTTTCCGGGCAGGTAGCGACCTCGTTATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGa                                    <  1:78942/71‑1 (MQ=255)
                       ttCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGa                                                     >  1:988347/1‑48 (MQ=255)
                        tCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTcgc                              <  1:3094188/70‑1 (MQ=255)
                        tCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTcgc                              <  1:2306985/70‑1 (MQ=255)
                        tCCGGGCAGGTAGCGACCTCGTTGATAGGTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTcgc                              <  1:2762675/69‑1 (MQ=255)
                                 gTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACAt                                            <  1:3406247/47‑1 (MQ=255)
                                 gTAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGa                                    <  1:1188609/55‑1 (MQ=255)
                                  tAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                    >  1:3317923/1‑70 (MQ=255)
                                  tAGCGACCTCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                    >  1:1139419/1‑70 (MQ=255)
                                   aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCggtggt                    >  1:1959556/1‑69 (MQ=255)
                                   aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGtt                   >  1:2847241/1‑70 (MQ=255)
                                   aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGtt                   >  1:3415104/1‑70 (MQ=255)
                                   aGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGtt                   >  1:783378/1‑70 (MQ=255)
                                          tCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtg                                           <  1:2758329/39‑1 (MQ=255)
                                          tCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtg                                           <  1:1224217/39‑1 (MQ=255)
                                          tCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtg                                           <  1:1559598/39‑1 (MQ=255)
                                          tCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtg                                           <  1:2193354/39‑1 (MQ=255)
                                          tCGTTGATAGGTTACGATGCCCTTCAGGAGTGCGACAtg                                           <  1:3611643/39‑1 (MQ=255)
                                                    gTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGt  >  1:429582/1‑70 (MQ=255)
                                                    gTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGt  >  1:3239120/1‑70 (MQ=255)
                                                    gTTACGATGCCCTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGt  >  1:2951115/1‑70 (MQ=255)
                                                               |                                                          
GATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTTGATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGT  >  W3110S.gb/85903‑86024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: