| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,194,164 | T→C | 100% | T416A (ACG→GCG) | rfaE ← | fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,194,164 | 0 | T | C | 91.2% | 101.1 / 1.5 | 34 | T416A (ACG→GCG) | rfaE | fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (17/14); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCG > W3110S.gb/3194104‑3194231 | tataGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTc > 1:3553659/1‑70 (MQ=255) gTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAAc < 1:3517255/70‑1 (MQ=255) gTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAAc < 1:258001/70‑1 (MQ=255) gTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTACGGCGTGTCCTCTTCAAAc < 1:2520494/70‑1 (MQ=255) tCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:1436851/71‑1 (MQ=255) gccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGaca > 1:101567/1‑71 (MQ=255) gccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGaca > 1:2843648/1‑71 (MQ=255) agcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGAc < 1:3229907/71‑1 (MQ=255) agcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGAc < 1:2072213/71‑1 (MQ=255) agcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGAc < 1:2417289/71‑1 (MQ=255) agcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCCCTTCAAACGACACTACCCAGTCGAc < 1:3280604/71‑1 (MQ=255) agATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTc < 1:1580229/41‑1 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt < 1:1487561/71‑1 (MQ=255) aTCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:945186/51‑1 (MQ=255) aTCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:2940921/51‑1 (MQ=255) tCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:1998089/50‑1 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGaca > 1:1948769/1‑51 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:2797632/1‑70 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:1986181/1‑70 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:1547310/1‑70 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:1438461/1‑70 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc > 1:3111196/1‑70 (MQ=255) tGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:980469/48‑1 (MQ=255) tGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGa < 1:293032/48‑1 (MQ=255) cAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTg > 1:552651/1‑71 (MQ=255) aTCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGcc < 1:1887265/71‑1 (MQ=255) aTCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGcc < 1:589539/71‑1 (MQ=255) aTCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGcc > 1:1463572/1‑71 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCa > 1:2656222/1‑39 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAg > 1:2989269/1‑54 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAg > 1:3579250/1‑54 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCATAATCa > 1:1113843/1‑71 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCa > 1:3280622/1‑71 (MQ=255) cAAGAGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAg > 1:404040/1‑54 (MQ=255) gcgcGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCg < 1:346503/71‑1 (MQ=255) gcgcGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCg < 1:3655692/71‑1 (MQ=255) | TATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCG > W3110S.gb/3194104‑3194231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |