| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,205,103 | A→G | 100% | intergenic (‑191/‑16) | ygiP ← / → ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,205,103 | 0 | A | G | 88.2% | 94.7 / 4.3 | 34 | intergenic (‑191/‑16) | ygiP/ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/1); new base G (11/19); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC > W3110S.gb/3205035‑3205172 | ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:1823213/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:3208910/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:3484939/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:2793713/71‑1 (MQ=255)ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:1864881/71‑1 (MQ=255)agTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:439445/70‑1 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa > 1:165081/1‑70 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa > 1:3548972/1‑70 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa > 1:748377/1‑70 (MQ=255) tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACgaa < 1:623675/69‑1 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTg < 1:2587271/71‑1 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTg < 1:2431920/71‑1 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTg < 1:2199025/71‑1 (MQ=255) tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGc < 1:1428247/71‑1 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGa < 1:1822408/71‑1 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGa < 1:1246902/71‑1 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGa < 1:2163474/71‑1 (MQ=255) gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGtaata < 1:165527/70‑1 (MQ=255) gTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:1044196/70‑1 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:1819209/62‑1 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:1911807/62‑1 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:482957/62‑1 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:2349958/62‑1 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:1654619/62‑1 (MQ=255) tgtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:614028/1‑69 (MQ=255) tgtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa > 1:1376091/1‑69 (MQ=255) tgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 1:3585023/71‑1 (MQ=255) ggTGAATTTTTCCAGACAAATACGAAAACTGGAGtt > 1:2429525/1‑36 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:3135186/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:3333843/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:346969/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:3473812/1‑70 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa > 1:50467/1‑70 (MQ=255) tttttCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt > 1:2402231/1‑69 (MQ=255) tttttCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt > 1:9639/1‑69 (MQ=255) cAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 1:17275/56‑1 (MQ=255) gACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg > 1:1282757/1‑56 (MQ=255) gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:1898086/47‑1 (MQ=255) aCAAATACGAAAACTTGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:1793439/46‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:283335/46‑1 (MQ=255) aCAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc < 1:1500941/46‑1 (MQ=255) aaaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa > 1:3625183/1‑67 (MQ=255) cAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTgac > 1:3320384/1‑69 (MQ=255) cAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc > 1:1633099/1‑71 (MQ=255) | GGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTC > W3110S.gb/3205035‑3205172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |