| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,349,295 | C→T | 61.8% | A124T (GCC→ACC) | mtgA ← | biosynthetic peptidoglycan transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,349,295 | 0 | C | T | 61.8% | 21.3 / 36.2 | 34 | A124T (GCC→ACC) | mtgA | biosynthetic peptidoglycan transglycosylase |
| Reads supporting (aligned to +/- strand): ref base C (11/2); new base T (7/14); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
CGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGG > W3110S.gb/3349238‑3349360 | cGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGaa < 1:3499495/71‑1 (MQ=255)cGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGaa < 1:2521617/71‑1 (MQ=255) gCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTgaga < 1:729664/61‑1 (MQ=255) gCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTgaga < 1:354334/61‑1 (MQ=255) cccTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAg < 1:2293995/71‑1 (MQ=255) ttttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGa < 1:1241034/66‑1 (MQ=255) ttCGCACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACcg > 1:2690599/1‑71 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACcg > 1:983663/1‑71 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACcg > 1:3204425/1‑71 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACcg > 1:1808905/1‑71 (MQ=255) tACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGttt > 1:3143489/1‑71 (MQ=255) cGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTc < 1:1576162/71‑1 (MQ=255) ccatcccatAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGc < 1:3110412/50‑1 (MQ=255) atcccatAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGaa < 1:752657/56‑1 (MQ=255) tcccatAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGtttt < 1:3681351/64‑1 (MQ=255) tcccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACcgcg < 1:298640/53‑1 (MQ=255) cccatAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGtttt < 1:814979/63‑1 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAg > 1:2748186/1‑44 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAg > 1:3525901/1‑44 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcgc > 1:906226/1‑70 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCt > 1:1653694/1‑71 (MQ=255) tAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGt < 1:708091/71‑1 (MQ=255) aaaaGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAg > 1:557312/1‑39 (MQ=255) aaaaGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCa > 1:2430797/1‑41 (MQ=255) aaaaGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTtg > 1:3196649/1‑71 (MQ=255) aaaaGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTtg > 1:1580610/1‑71 (MQ=255) aaaaGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTtg > 1:2109279/1‑71 (MQ=255) gaTTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTgcgc > 1:347542/1‑70 (MQ=255) gaTTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTgcgc > 1:1014475/1‑70 (MQ=255) gaTTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGcc < 1:2079535/71‑1 (MQ=255) tttGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCa > 1:2163561/1‑49 (MQ=255) tttGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCa > 1:2295991/1‑49 (MQ=255) ttGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAggg < 1:2738028/70‑1 (MQ=255) ttGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGAGCGCCAggg < 1:3709364/70‑1 (MQ=255) | CGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGG > W3110S.gb/3349238‑3349360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |