| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,358,271 | A→G | 100% | K1262R (AAA→AGA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,358,271 | 0 | A | G | 92.9% | 84.9 / ‑1.2 | 28 | K1262R (AAA→AGA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (16/10); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGCC > W3110S.gb/3358203‑3358338 | tGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCagaa > 1:1880131/1‑71 (MQ=255) aTAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAAcc < 1:2130303/71‑1 (MQ=255) aTAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAAcc < 1:3040496/71‑1 (MQ=255) ctgctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCTATGAGCGCCAGAGCAGAACCTTCTGGt > 1:1959601/1‑70 (MQ=255) ctgctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGt > 1:1429661/1‑70 (MQ=255) gctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTc > 1:3503826/1‑70 (MQ=255) ctgAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa < 1:642990/71‑1 (MQ=255) cgcAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGc < 1:985922/71‑1 (MQ=255) cgcAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGc < 1:3132966/71‑1 (MQ=255) gctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACacc > 1:640242/1‑70 (MQ=255) aaCAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGa < 1:3568108/52‑1 (MQ=255) aCAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGtt > 1:1295861/1‑71 (MQ=255) aCAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGtt > 1:190665/1‑71 (MQ=255) aGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCt > 1:2797763/1‑71 (MQ=255) aGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCt > 1:1836821/1‑71 (MQ=255) aCCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGgcg > 1:3451402/1‑71 (MQ=255) aCCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACAACGACCGTTCTGTCGgc > 1:2295933/1‑70 (MQ=255) gTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTc < 1:2604626/37‑1 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt < 1:2532710/70‑1 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCttt > 1:36368/1‑70 (MQ=255) gAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAgg > 1:2279616/1‑71 (MQ=255) aGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc > 1:1127968/1‑71 (MQ=255) aGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc > 1:2222387/1‑71 (MQ=255) aGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc > 1:3321371/1‑71 (MQ=255) gcgcCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc < 1:285310/70‑1 (MQ=255) gcCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCtat < 1:3277764/71‑1 (MQ=255) agagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATc > 1:2759746/1‑71 (MQ=255) gagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCtat < 1:2246924/67‑1 (MQ=255) gCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGcc > 1:3184897/1‑71 (MQ=255) | TGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCGCC > W3110S.gb/3358203‑3358338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |