| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,364,443 | T→C | 12.1% | L594L (CTT→CTC) | yhcD → | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,364,443 | 0 | T | C | 12.1% | 221.6 / 19.7 | 84 | L594L (CTT→CTC) | yhcD | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (38/35); new base C (4/6); total (43/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTGGTATTGCCGTACAT > W3110S.gb/3364375‑3364512 | cACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGc > 1:3279006/1‑71 (MQ=255)cACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGc > 1:2938234/1‑71 (MQ=255)cACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGc > 1:2397828/1‑71 (MQ=255)cACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGc > 1:2241391/1‑71 (MQ=255) gacgaACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGc < 1:1661724/71‑1 (MQ=255) gTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTCGCCGCAGGAGCa < 1:1130731/71‑1 (MQ=255) gTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTCGCCGCAGGAGCa < 1:3298018/71‑1 (MQ=255) gTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTCGCCGCAGGAGCa < 1:2468175/71‑1 (MQ=255) gAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAg > 1:3463460/1‑71 (MQ=255) aCGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCgg < 1:1251776/71‑1 (MQ=255) cTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:2530977/71‑1 (MQ=255) cTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:2487030/71‑1 (MQ=255) cTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:2296336/71‑1 (MQ=255) ttGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:995446/70‑1 (MQ=255) gAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGa < 1:306342/69‑1 (MQ=255) aCGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGc < 1:3042098/71‑1 (MQ=255) cAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTa < 1:2608571/71‑1 (MQ=255) cAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTa < 1:1144436/71‑1 (MQ=255) gTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:743628/60‑1 (MQ=255) gTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:3163410/60‑1 (MQ=255) gTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:1295587/60‑1 (MQ=255) gTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:1099487/60‑1 (MQ=255) gTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:2230309/60‑1 (MQ=255) gTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTg < 1:36234/60‑1 (MQ=255) aTCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGAt > 1:2805263/1‑60 (MQ=255) aTCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACtt > 1:1624916/1‑70 (MQ=255) aTCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:2405692/1‑71 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAg > 1:1709967/1‑55 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACtt > 1:201436/1‑69 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:283778/1‑70 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:3154207/1‑70 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:3568751/1‑70 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:471021/1‑70 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:1299069/1‑70 (MQ=255) tCAGTTCCAATCTCCATTACACCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTa > 1:1275670/1‑70 (MQ=255) aGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACaa > 1:165584/1‑71 (MQ=255) aGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACaa > 1:3201648/1‑71 (MQ=255) aGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACaa > 1:1690668/1‑71 (MQ=255) aGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACaa > 1:260522/1‑71 (MQ=255) aGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACaa > 1:258003/1‑71 (MQ=255) ttCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGt < 1:2955401/52‑1 (MQ=255) tCCAATCTCCATTACACCCAAGTTAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACgg > 1:1663608/1‑71 (MQ=255) tCCAATATCCATTACACCCAAGTTAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACgg > 1:3509/1‑71 (MQ=255) ccAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGa > 1:2505650/1‑52 (MQ=255) ccAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACgg > 1:3294005/1‑70 (MQ=255) ccAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACgg > 1:2137276/1‑70 (MQ=255) ccAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACgg > 1:3325128/1‑70 (MQ=255) ccAATCTCCATTACACACAAGTCAGCCTAGCCCCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACg > 1:3631840/1‑69 (MQ=255) aaTCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTAc < 1:399987/71‑1 (MQ=255) tctcCATTACACCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTAc < 1:2708497/61‑1 (MQ=255) tcCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATg > 1:280824/1‑71 (MQ=255) tcCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATg > 1:2037093/1‑71 (MQ=255) tcCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATg > 1:183796/1‑71 (MQ=255) cATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:1282860/1‑67 (MQ=255) cATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:2190656/1‑67 (MQ=255) cATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:2865908/1‑67 (MQ=255) cATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:2138316/1‑67 (MQ=255) ttACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAg > 1:2838209/1‑71 (MQ=255) acacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:1054059/1‑63 (MQ=255) acacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:2768343/1‑63 (MQ=255) acacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:265686/1‑63 (MQ=255) acacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGa > 1:2438764/1‑63 (MQ=255) acacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAgg < 1:709279/70‑1 (MQ=255) acacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAgg < 1:1496496/70‑1 (MQ=255) acacCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggt > 1:2630721/1‑71 (MQ=255) acacCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggt > 1:1142396/1‑71 (MQ=255) acacCCAACTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACg > 1:1559791/1‑62 (MQ=255) cacCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggt < 1:1320252/70‑1 (MQ=255) acCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtgg < 1:3561038/71‑1 (MQ=255) acCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtgg < 1:764899/71‑1 (MQ=255) acCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtgg < 1:1833594/71‑1 (MQ=255) acCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtgg < 1:2988728/71‑1 (MQ=255) acCCAAGTCAGCCTT‑CCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtggt < 1:3238981/71‑1 (MQ=255) acCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtgg < 1:2238896/71‑1 (MQ=255) acCCAAGTCAGCCTCGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtgg < 1:47157/71‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGt < 1:20724/63‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGt < 1:3497093/63‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGt < 1:2498727/63‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGt < 1:2598216/63‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGt < 1:1585116/63‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGt < 1:120987/63‑1 (MQ=255) cccAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAggtggt < 1:970929/71‑1 (MQ=255) aGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTGGTATTGCCg < 1:1446097/70‑1 (MQ=255) aGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTGGTATTGCCg < 1:2095659/70‑1 (MQ=255) ttGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTGGTATTGCCGTACAt > 1:663806/1‑71 (MQ=255) | CACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTGGTATTGCCGTACAT > W3110S.gb/3364375‑3364512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |