| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,365,218 | A→G | 63.4% | pseudogene (179/480 nt) pseudogene (179/1745 nt) |
yhcE → yhcE → |
ECK3207:JW3184:b3217; hypothetical protein, N‑ter fragment ECK3207:JW3184+JW3187:b4569; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,365,218 | 0 | A | G | 63.4% | 39.4 / 38.4 | 41 | pseudogene (179/480 nt) pseudogene (179/1745 nt) | yhcE yhcE | ECK3207:JW3184:b3217; hypothetical protein, N‑ter fragment ECK3207:JW3184+JW3187:b4569; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/11); new base G (13/13); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGA > W3110S.gb/3365152‑3365285 | ccATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatgcgatgc < 1:3454508/71‑1 (MQ=255)ccATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatgcgatgc < 1:1135548/71‑1 (MQ=255)ccATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatgcgatgc < 1:1481663/71‑1 (MQ=255) gcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAg < 1:42745/71‑1 (MQ=255) gcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAg < 1:1624172/71‑1 (MQ=255) gcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAg < 1:1326758/71‑1 (MQ=255) gcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAg < 1:3440186/71‑1 (MQ=255) cGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTaatgcgatgc < 1:3620511/54‑1 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCata > 1:1062502/1‑63 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGAT‑AGGACGCTCata > 1:3037198/1‑62 (MQ=255) aacaacACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTc < 1:1431467/71‑1 (MQ=255) aacaacACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTc < 1:3437907/71‑1 (MQ=255) tAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATAcc > 1:1249614/1‑71 (MQ=255) tAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATAcc > 1:2717870/1‑71 (MQ=255) aGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCg > 1:1261226/1‑71 (MQ=255) aGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCg > 1:1652123/1‑71 (MQ=255) gCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAg < 1:1831199/39‑1 (MQ=255) cagacagaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:3404452/71‑1 (MQ=255) cagacagaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:2063457/71‑1 (MQ=255) cagacagaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:3008040/71‑1 (MQ=255) cagacagaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:1115081/71‑1 (MQ=255) cagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAata > 1:3103056/1‑71 (MQ=255) cagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAata > 1:2253380/1‑71 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAatat < 1:2908974/71‑1 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAatat < 1:3563694/71‑1 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAatat < 1:1301333/71‑1 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAata > 1:3574158/1‑70 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAata > 1:3283124/1‑70 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAat > 1:3089118/1‑69 (MQ=255) aaCAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:2306734/55‑1 (MQ=255) aaCAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:2841082/55‑1 (MQ=255) aaCAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:914185/55‑1 (MQ=255) aaCAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:1718991/55‑1 (MQ=255) aaCAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:1737206/55‑1 (MQ=255) aaCAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:622800/55‑1 (MQ=255) aaTCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTcc > 1:84294/1‑70 (MQ=255) aaTCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTcc > 1:3575454/1‑70 (MQ=255) aaTCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTcc > 1:2950855/1‑70 (MQ=255) aCGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg > 1:727751/1‑45 (MQ=255) aCGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg > 1:2128187/1‑45 (MQ=255) aCGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg > 1:2176970/1‑45 (MQ=255) gCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACg < 1:2336797/71‑1 (MQ=255) gCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACg < 1:2738942/71‑1 (MQ=255) gCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACg < 1:1936745/71‑1 (MQ=255) gCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACg < 1:1549409/71‑1 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTaa < 1:1343862/50‑1 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTaa < 1:1063587/50‑1 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatttg > 1:2610822/1‑70 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatttg > 1:2173988/1‑70 (MQ=255) tgcgatgcgaTCAGGACGCTCATATTCGTGTTCAGTTGaa < 1:3230108/40‑1 (MQ=255) tgcgatgcgaTCAGGACGCTCATATTCGTGTTCAGTTGaa < 1:2477007/40‑1 (MQ=255) tgcgatgcgaTCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCca < 1:3623678/57‑1 (MQ=255) tgcgatgcgaTCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCca < 1:2556605/57‑1 (MQ=255) tgcgatgcgaTCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatttgga < 1:929100/71‑1 (MQ=255) | CCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGA > W3110S.gb/3365152‑3365285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |