| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,381,341 | T→C | 9.3% | S248S (AGT→AGC) | degQ → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,381,341 | 0 | T | C | 9.3% | 115.7 / 4.4 | 43 | S248S (AGT→AGC) | degQ | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base T (18/21); new base C (2/2); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
AACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGCATCA > W3110S.gb/3381282‑3381411 | aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:3122528/1‑70 (MQ=255)aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:464645/1‑70 (MQ=255)aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:2418505/1‑70 (MQ=255)aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgcgc > 1:3646384/1‑70 (MQ=255)aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg < 1:3422909/71‑1 (MQ=255) cacTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGaa < 1:1675760/71‑1 (MQ=255) acTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGACATCCCCAGTAATATGGCGCGAac < 1:2264188/71‑1 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1441645/58‑1 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1561023/58‑1 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:3440094/58‑1 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:3466969/58‑1 (MQ=255) gCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAata > 1:517748/1‑58 (MQ=255) gCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGCAata > 1:418151/1‑58 (MQ=255) aaTCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:153497/54‑1 (MQ=255) aaTCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1725132/54‑1 (MQ=255) aaTCATTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:1415377/54‑1 (MQ=255) tCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:2544808/52‑1 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAata > 1:3634196/1‑52 (MQ=255) gggATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAAt > 1:2347191/1‑71 (MQ=255) attTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAAcgcg < 1:3518317/71‑1 (MQ=255) tttGCCATCCCCAGTAATATGGCGGGAACACTGGCgca < 1:3641210/38‑1 (MQ=255) tttGCCATCCCCAGTAATATGGCGCGAACACTGGCgca < 1:29948/38‑1 (MQ=255) tGCCATCCCCAGTAATATGGCGCGAACACTGGCgcagc > 1:2970124/1‑38 (MQ=255) tGCCATCCCCAGTAATATGGCGCGAACACTGGCgcagc > 1:1704145/1‑38 (MQ=255) cATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:1224439/71‑1 (MQ=255) cATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:491170/71‑1 (MQ=255) cATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:2137983/71‑1 (MQ=255) cATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:1747428/71‑1 (MQ=255) cATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:1560213/71‑1 (MQ=255) cATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGt < 1:78829/71‑1 (MQ=255) tCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCa < 1:2309288/57‑1 (MQ=255) tCCCCAGCAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCa < 1:677008/57‑1 (MQ=255) ccccAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTa > 1:751419/1‑70 (MQ=255) cccAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAgg > 1:624110/1‑71 (MQ=255) cccAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAgg > 1:1079200/1‑71 (MQ=255) cAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCa < 1:1689782/53‑1 (MQ=255) aGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:972690/1‑54 (MQ=255) aGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:3627156/1‑54 (MQ=255) aGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:2772507/1‑54 (MQ=255) aGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:2039847/1‑54 (MQ=255) gTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:2864394/1‑53 (MQ=255) gTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:1494627/1‑53 (MQ=255) gTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCaaa > 1:1350653/1‑53 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACtt > 1:3179436/1‑39 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGc < 1:3622454/67‑1 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGc < 1:3008081/67‑1 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGc < 1:1678063/67‑1 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGc < 1:141194/67‑1 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGCATc > 1:3415299/1‑70 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGCATc > 1:912729/1‑70 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGCATCa < 1:2865610/71‑1 (MQ=255) tAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACG‑GGTTTGTTAGGCATCa > 1:1580064/1‑70 (MQ=255) | AACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGGTTTGTTAGGCATCA > W3110S.gb/3381282‑3381411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |