| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,404,820 | G→A | 73.5% | intergenic (+507/‑471) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,404,820 | 0 | G | A | 73.5% | 49.7 / 21.2 | 34 | intergenic (+507/‑471) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/3); new base A (13/12); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01 | |||||||||||
TCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGAT > W3110S.gb/3404760‑3404884 | tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAtat > 1:734678/1‑69 (MQ=255)tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt > 1:3240171/1‑71 (MQ=255) ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc > 1:2463006/1‑71 (MQ=255) ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc > 1:3245215/1‑71 (MQ=255) gcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAgg < 1:316230/71‑1 (MQ=255) gcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAgg < 1:1811012/71‑1 (MQ=255) gcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAgg < 1:1734399/71‑1 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:494424/1‑71 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcag < 1:1178330/70‑1 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:3651109/1‑71 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:1950723/1‑71 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:2235273/1‑71 (MQ=255) attattTGTGGTCTGTGAAGATTATCTCATTGCAACCCCt < 1:1916198/40‑1 (MQ=255) attattTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagctttt < 1:1162667/70‑1 (MQ=255) attattTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagctttt < 1:3043820/70‑1 (MQ=255) attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCATGGCTGGCTTTTCAGCTTTTCAcc > 1:3004200/1‑71 (MQ=255) gtgGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTa < 1:3297522/70‑1 (MQ=255) gtgGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTa < 1:812299/70‑1 (MQ=255) gtgGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTa < 1:3194930/70‑1 (MQ=255) tgAAGATTATCTCATTGCAACCCCTCATCTTCGCAgg > 1:3197811/1‑37 (MQ=255) tgAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat > 1:1085374/1‑68 (MQ=255) aGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag < 1:1699634/71‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:326699/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGAAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:1818049/68‑1 (MQ=255) aTTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:1719141/68‑1 (MQ=255) aTTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:3675161/71‑1 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCAcc > 1:1233908/1‑43 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTAc > 1:1876499/1‑47 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg > 1:2813664/1‑48 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg > 1:2147952/1‑48 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg > 1:3266543/1‑48 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg > 1:3346093/1‑48 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg > 1:1462116/1‑48 (MQ=255) gCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg > 1:1028426/1‑48 (MQ=255) | TCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGAT > W3110S.gb/3404760‑3404884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |