| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,447,843 | A→G | 76.5% | V1163V (GTT→GTC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,447,843 | 0 | A | G | 76.5% | 99.4 / 28.0 | 51 | V1163V (GTT→GTC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (4/8); new base G (17/22); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGCAA > W3110S.gb/3447775‑3447910 | tACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:1018742/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa < 1:1308855/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa < 1:2682508/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa < 1:2548153/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa < 1:3005727/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa < 1:1738025/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGa < 1:1343014/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGa < 1:1548160/71‑1 (MQ=255) cGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGa < 1:1576260/71‑1 (MQ=255) ggATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGa < 1:2210568/70‑1 (MQ=255) cTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCt < 1:740823/71‑1 (MQ=255) cTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCt < 1:2097926/71‑1 (MQ=255) cTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCt < 1:3043493/71‑1 (MQ=255) tCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTc < 1:2374225/71‑1 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTAGCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:700654/1‑71 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:2287215/71‑1 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:350197/71‑1 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:341890/71‑1 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAg < 1:2209004/71‑1 (MQ=255) gggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:2193820/70‑1 (MQ=255) aTAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATAcag > 1:197969/1‑49 (MQ=255) aTAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:187588/1‑65 (MQ=255) aTAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:2513635/1‑65 (MQ=255) aTAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:3452886/1‑65 (MQ=255) aTAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGc > 1:2829320/1‑71 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCTAAGGAGAc < 1:1203226/41‑1 (MQ=38) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:667493/41‑1 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGa > 1:3596306/1‑64 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGa > 1:1844061/1‑64 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGa > 1:1557854/1‑64 (MQ=255) acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:1668842/71‑1 (MQ=255) tttACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCt < 1:70011/39‑1 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:2234517/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:1047697/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:1525708/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:3396618/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:3289231/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:1821218/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:2887963/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:2749240/1‑71 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGAt > 1:2773008/1‑40 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGAt > 1:188373/1‑40 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGAt > 1:223973/1‑40 (MQ=255) tCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGc < 1:2552807/48‑1 (MQ=255) tCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGc < 1:572836/48‑1 (MQ=255) tCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGaa > 1:1386404/1‑71 (MQ=255) aaGGAGACGATACTGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGc < 1:242906/71‑1 (MQ=255) aaGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGc < 1:3411231/71‑1 (MQ=255) aaGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGc < 1:96774/71‑1 (MQ=255) ggAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGCaa < 1:3321145/71‑1 (MQ=255) ggAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGCaa < 1:2449621/71‑1 (MQ=255) | TACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGGTCCGCAA > W3110S.gb/3447775‑3447910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |