| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,573,836 | A→G | 90.3% | V200A (GTG→GCG) | yihN ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,573,836 | 0 | A | G | 90.3% | 121.0 / 4.6 | 41 | V200A (GTG→GCG) | yihN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (20/17); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CAGATAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACGGCAATCAC > W3110S.gb/3573770‑3573903 | cAGATAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTc < 1:2940983/71‑1 (MQ=255)cAGATAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTc < 1:538086/71‑1 (MQ=255) aTAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAac > 1:2004304/1‑71 (MQ=255) aTAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAac > 1:2319736/1‑71 (MQ=255) ttttGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggac < 1:3160690/71‑1 (MQ=255) ttttGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggac < 1:1965962/71‑1 (MQ=255) ttGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacgg > 1:112117/1‑71 (MQ=255) ttGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacg < 1:707558/70‑1 (MQ=255) ttGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacg < 1:3648212/70‑1 (MQ=255) gTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGt < 1:96936/70‑1 (MQ=255) gAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTc < 1:691882/71‑1 (MQ=255) gTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCAtt < 1:718923/71‑1 (MQ=255) aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacgg > 1:3151742/1‑50 (MQ=255) aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacgg > 1:2955059/1‑50 (MQ=255) aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacgg > 1:2872187/1‑50 (MQ=255) aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacgg > 1:1401015/1‑50 (MQ=255) aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCa > 1:1336990/1‑70 (MQ=255) aTACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAg > 1:68580/1‑71 (MQ=255) tACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTc < 1:3032778/40‑1 (MQ=255) cTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCGcc > 1:2329670/1‑36 (MQ=255) ggTGTTTTTCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACa > 1:2533245/1‑70 (MQ=255) tttttCGATGCTCCGTCTTCTTTTTTCGCCTCAacggacg > 1:494633/1‑40 (MQ=255) tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTcttctt > 1:2439248/1‑50 (MQ=255) tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaaaa > 1:1232902/1‑71 (MQ=255) tttCGATGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGaa > 1:1630220/1‑69 (MQ=255) tGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa > 1:3659035/1‑71 (MQ=255) tGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa < 1:2648508/71‑1 (MQ=255) tGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa < 1:170107/71‑1 (MQ=255) tGCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa < 1:135235/71‑1 (MQ=255) gCTCCGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataa > 1:803347/1‑67 (MQ=255) cGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataataa < 1:2297741/66‑1 (MQ=255) cGTCTTCTTTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGcc < 1:3090868/70‑1 (MQ=255) ttcttTTTTCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACgg > 1:2773813/1‑71 (MQ=255) ttcttTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAaataa > 1:3544869/1‑59 (MQ=255) ttcttTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACg > 1:1454783/1‑70 (MQ=255) cttTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACg < 1:1579566/68‑1 (MQ=255) ttttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACGGCAAt < 1:2548989/70‑1 (MQ=255) ttttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACGGCAAt < 1:2481585/70‑1 (MQ=255) ttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCa > 1:1227177/1‑37 (MQ=255) ttCGCCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCt > 1:671347/1‑60 (MQ=255) tCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACGGCAATcac < 1:157137/70‑1 (MQ=255) | CAGATAGTTTTGTCTTTCAGCACCGAGGTCATACTGGTGTTTTTCGATGCTCCGTCTTCTTTTTTCACCTCAACGGACGGTGCCTCTTCTTTGTCATTCAGGACAAAGAAAATAATAATGCCTACGGCAATCAC > W3110S.gb/3573770‑3573903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |