| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,622,543 | C→T | 53.3% | A362A (GCG→GCA) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,622,543 | 0 | C | T | 53.3% | ‑0.9 / 39.7 | 30 | A362A (GCG→GCA) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (11/3); new base T (10/6); total (21/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCA > W3110S.gb/3622476‑3622604 | tAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAgtgc > 1:923018/1‑70 (MQ=255) gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:965675/71‑1 (MQ=255) gacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:1753747/71‑1 (MQ=255) gTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGt > 1:1057492/1‑71 (MQ=255) gTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagca > 1:65562/1‑69 (MQ=255) gTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:1085098/1‑71 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGc > 1:595666/1‑70 (MQ=255) gAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGt > 1:1692297/1‑71 (MQ=255) gcgcTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCa < 1:840039/71‑1 (MQ=255) tgcTCTACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCAt < 1:1803422/70‑1 (MQ=255) tgcTCTACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCAt < 1:2709329/70‑1 (MQ=255) tgcTCTACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCAt < 1:3722063/70‑1 (MQ=255) aCTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGt < 1:3572129/71‑1 (MQ=255) ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:1837899/1‑71 (MQ=255) ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:1869798/1‑71 (MQ=255) ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:2551434/1‑71 (MQ=255) ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:3454328/1‑71 (MQ=255) ctGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAgg > 1:3375857/1‑71 (MQ=255) gCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAgg > 1:991951/1‑70 (MQ=255) gCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAgg > 1:3347272/1‑70 (MQ=255) gCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAgg > 1:2413078/1‑70 (MQ=255) gCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAgg > 1:1128997/1‑70 (MQ=255) gCTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAg > 1:331881/1‑69 (MQ=255) cTGCCGTGTCACCACTGTTCAGTGCATCGCGCAGCGGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:2577553/71‑1 (MQ=255) gCCGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCaaa > 1:1635097/1‑71 (MQ=255) ccGTGTCACCACTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCAt < 1:2071348/50‑1 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:3656871/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:214866/1‑71 (MQ=255) accacTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCttt > 1:952861/1‑71 (MQ=255) acTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCa > 1:3161848/1‑71 (MQ=255) acTGTTCAGTGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCa > 1:3101936/1‑71 (MQ=255) | TAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCA > W3110S.gb/3622476‑3622604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |