| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,682,573 | G→A | 100% | L211L (CTG→TTG) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,682,573 | 0 | G | A | 87.5% | 102.1 / 7.4 | 40 | L211L (CTG→TTG) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (12/23); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGGAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCG > W3110S.gb/3682505‑3682635 | aaGGAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAgcg > 1:1535465/1‑71 (MQ=255)aaGGAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAgcg > 1:1576329/1‑71 (MQ=255)aaGGAACAGCTGATTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAgcg > 1:1449813/1‑71 (MQ=255) ggAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAacg < 1:951229/69‑1 (MQ=255) ggAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAacg < 1:805999/69‑1 (MQ=255) ggAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAacg < 1:3327390/69‑1 (MQ=255) ggAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAacg < 1:3417773/69‑1 (MQ=255) aaCAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTc > 1:441148/1‑71 (MQ=255) aaCAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGAGACAGGCCCAGCGCTTc > 1:2575936/1‑71 (MQ=255) aGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGt > 1:2424045/1‑71 (MQ=255) aGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGt > 1:175669/1‑71 (MQ=255) ctTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAgg < 1:2196695/70‑1 (MQ=255) ctTACGGGCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAgg < 1:3345415/70‑1 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:450244/71‑1 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:238680/71‑1 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:1996780/71‑1 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:2646339/71‑1 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:89952/71‑1 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGt > 1:1033437/1‑70 (MQ=255) cGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCAGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:581930/71‑1 (MQ=255) ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc < 1:3185952/71‑1 (MQ=255) ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc < 1:21987/71‑1 (MQ=255) ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc < 1:3543221/71‑1 (MQ=255) ggTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTc < 1:1482781/71‑1 (MQ=255) cGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGtt < 1:1107378/64‑1 (MQ=255) tGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCgg > 1:2748801/1‑71 (MQ=255) ccAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTa < 1:1231399/71‑1 (MQ=255) ccgcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAAc > 1:702788/3‑70 (MQ=255) cagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCg > 1:3101797/1‑40 (MQ=255) cagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAg > 1:978199/1‑45 (MQ=255) cagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACa > 1:739279/1‑71 (MQ=255) cagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACa > 1:2915094/1‑71 (MQ=255) cagcGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACa > 1:1765210/1‑71 (MQ=255) agcGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt > 1:3620125/1‑71 (MQ=255) gCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:3370093/71‑1 (MQ=255) gCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:1372651/71‑1 (MQ=255) ttGCCCGGCTGCGACAGGCCCAACGCTTCGGTCTGGCAGTTCATTGAGTTAGCGGTa < 1:1337955/57‑1 (MQ=255) ttGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:1728287/46‑1 (MQ=255) ttGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:3044200/46‑1 (MQ=255) tGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:3138993/45‑1 (MQ=255) tGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAg > 1:14403/1‑71 (MQ=255) cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:3693855/64‑1 (MQ=255) cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGa < 1:3529535/71‑1 (MQ=255) cTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGc < 1:1916173/62‑1 (MQ=255) tGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCg > 1:1642649/1‑69 (MQ=255) tGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCg > 1:1878844/1‑69 (MQ=255) aGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCg > 1:1614914/1‑70 (MQ=255) | AAGGAACAGCTGCTTACGGTCGGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCG > W3110S.gb/3682505‑3682635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |