| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,705,799 | A→G | 100% | E70E (GAA→GAG) | yieN → | fused predicted transcriptional regulators |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,705,799 | 0 | A | G | 76.9% | 20.2 / 2.9 | 13 | E70E (GAA→GAG) | yieN | fused predicted transcriptional regulators |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (5/5); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGCGCTAAAAGATG > W3110S.gb/3705735‑3705869 | gTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtg < 1:176857/71‑1 (MQ=255) tATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtga > 1:296913/1‑71 (MQ=255) aTTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtgat > 1:97478/1‑71 (MQ=255) aTTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCtgat > 1:1444884/1‑71 (MQ=255) ttGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtgat < 1:320899/70‑1 (MQ=255) ttGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtgat < 1:750263/70‑1 (MQ=255) gCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCTGATGAc > 1:1198755/1‑71 (MQ=255) gCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCtgatgcc > 1:49214/1‑69 (MQ=255) cGCCTTTCAGAATGCCCGAGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGt > 1:3109364/1‑70 (MQ=255) cAGAATGCCCGCGCGTTTGAGTATCTGATGCCCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTc > 1:3578282/1‑71 (MQ=255) aGAATGCCCGCGCGTTTGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTc < 1:1399023/70‑1 (MQ=255) aGAATGCCCGCGCGTTTGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTc < 1:2399136/70‑1 (MQ=255) aTGCCCGCGCGTTTGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTAt > 1:2829840/1‑70 (MQ=255) aaTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGCGCTAAAAGAt < 1:1918094/71‑1 (MQ=255) atatCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGCGCTAAAAGATg < 1:2751587/71‑1 (MQ=255) atatCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGCGCTAAAAGATg < 1:3701235/71‑1 (MQ=255) | GTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGCGCTAAAAGATG > W3110S.gb/3705735‑3705869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |