Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,709,115 C→T 16.0% E138E (GAG→GAA)  asnA ← asparagine synthetase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,709,1150CT16.0% 123.8 / 15.5 50E138E (GAG→GAA) asnAasparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base T (2/6);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACT  >  W3110S.gb/3709059‑3709182
                                                        |                                                                   
aGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtttt                                                       <  1:2923546/71‑1 (MQ=255)
    cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                     >  1:913615/1‑69 (MQ=255)
    cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                     >  1:3670144/1‑69 (MQ=255)
    cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                     >  1:3164118/1‑69 (MQ=255)
    cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                     >  1:1855241/1‑69 (MQ=255)
    cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                     >  1:2776691/1‑69 (MQ=255)
    cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                     >  1:1684968/1‑69 (MQ=255)
        ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTc                                                >  1:1927973/1‑70 (MQ=255)
        ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTc                                                >  1:1783202/1‑70 (MQ=255)
        ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTc                                                >  1:1485784/1‑70 (MQ=255)
             cGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCgag                                             <  1:2636398/68‑1 (MQ=255)
             cGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCgag                                             <  1:1429285/68‑1 (MQ=255)
             cGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGaa                                           <  1:2585722/70‑1 (MQ=255)
              gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                         <  1:1530305/71‑1 (MQ=255)
              gCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAAtt                                         <  1:1940158/71‑1 (MQ=255)
               cTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAAtt                                         <  1:2265278/70‑1 (MQ=255)
                     gCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTc                                  <  1:737963/71‑1 (MQ=255)
                        gCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCAcc                               <  1:3508179/71‑1 (MQ=255)
                        gCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCAcc                               <  1:2286647/71‑1 (MQ=255)
                         cTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCgag                                             <  1:222305/56‑1 (MQ=255)
                         cTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCgag                                             <  1:2160138/56‑1 (MQ=255)
                         cTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCgag                                             <  1:1341644/56‑1 (MQ=255)
                           tCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGaa                                           >  1:1246906/1‑56 (MQ=255)
                           tCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGt                             >  1:2696445/1‑70 (MQ=255)
                           tCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTc                            >  1:14887/1‑71 (MQ=255)
                              gTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGcc                         <  1:2634594/71‑1 (MQ=255)
                              gTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGcc                         <  1:2746415/71‑1 (MQ=255)
                               ttGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGGCGcc                         <  1:3637153/70‑1 (MQ=255)
                                  cTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCAGCGTCGc                          <  1:478403/66‑1 (MQ=255)
                                  cTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGc                          <  1:2904611/66‑1 (MQ=255)
                                  cTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGc                          <  1:653644/66‑1 (MQ=255)
                                  cTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCAt                      <  1:492322/70‑1 (MQ=255)
                                  cTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCAt                      <  1:344384/70‑1 (MQ=255)
                                       aTTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCAt                      <  1:2468518/65‑1 (MQ=255)
                                          cccGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTc             >  1:789559/1‑71 (MQ=255)
                                           ccGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc            <  1:76742/71‑1 (MQ=255)
                                            cGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc           <  1:709542/71‑1 (MQ=255)
                                            cGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc           <  1:1112294/71‑1 (MQ=255)
                                            cGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc           <  1:1061719/71‑1 (MQ=255)
                                              cccAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTAc                   <  1:3164504/61‑1 (MQ=255)
                                              cccAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTAc                   <  1:1347500/61‑1 (MQ=255)
                                              cccAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTAc                   <  1:1345073/61‑1 (MQ=255)
                                                    tCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCa    >  1:99490/1‑70 (MQ=255)
                                                    tCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCAc   >  1:2784063/1‑71 (MQ=255)
                                                    tCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCAc   >  1:2608966/1‑71 (MQ=255)
                                                    tCGCATCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCAc   >  1:657810/1‑71 (MQ=255)
                                                     cGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACt  >  1:824347/1‑71 (MQ=255)
                                                     cGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGcc                         >  1:1477592/1‑48 (MQ=255)
                                                     cGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTa                    >  1:1795450/1‑53 (MQ=255)
                                                     cGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACt  >  1:1227894/1‑71 (MQ=255)
                                                        |                                                                   
AGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCACT  >  W3110S.gb/3709059‑3709182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑