| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 230,040 | C→A | 100% | R281L (CGC→CTC) | yafC ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 230,040 | 0 | C | A | 84.1% | 80.8 / 18.2 | 44 | R281L (CGC→CTC) | yafC | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/7); new base A (17/20); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTCTGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTT > W3110S.gb/229974‑230103 | tctctGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGagcg > 1:2572850/1‑70 (MQ=255) tctGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTAc > 1:3676566/1‑71 (MQ=255) tctGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTAc > 1:3336587/1‑71 (MQ=255) tctGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTAc > 1:2696174/1‑71 (MQ=255) tctGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTAc > 1:1713130/1‑71 (MQ=255) ctGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACt > 1:1613739/1‑71 (MQ=255) tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTa < 1:1251536/68‑1 (MQ=255) tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACt > 1:34650/1‑70 (MQ=255) cAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTAcc > 1:1384474/1‑71 (MQ=255) cAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTAc > 1:1362478/1‑70 (MQ=255) aGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCg > 1:821575/1‑71 (MQ=255) tccGGTAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt < 1:1346950/57‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:186712/71‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:537932/71‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGt < 1:2205719/46‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGt < 1:2822271/46‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTaa < 1:2196259/68‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:1698694/70‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:3166251/70‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:751120/70‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:1204028/70‑1 (MQ=255) tttttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:343906/70‑1 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGAc > 1:3005967/1‑71 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGAc > 1:2276338/1‑71 (MQ=255) aCATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg < 1:1604401/70‑1 (MQ=255) tGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:3542542/62‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:326941/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:1073990/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:236296/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGAAGGCACTg < 1:780121/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:3507858/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:1550112/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGa < 1:160107/71‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGa < 1:2094803/71‑1 (MQ=255) aaTCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTcc < 1:2519552/71‑1 (MQ=255) aaTCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTcc < 1:3165110/71‑1 (MQ=255) aTCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc < 1:1500827/69‑1 (MQ=255) cGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCa > 1:3488413/1‑69 (MQ=255) cGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCa > 1:2869833/1‑69 (MQ=255) cGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCa > 1:79610/1‑69 (MQ=255) tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTgg > 1:1424904/1‑70 (MQ=255) tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTgg > 1:3103133/1‑70 (MQ=255) aaGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAac < 1:281788/71‑1 (MQ=255) cGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACtt > 1:1832753/1‑70 (MQ=255) ggATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg < 1:601650/39‑1 (MQ=255) | TCTCTGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTT > W3110S.gb/229974‑230103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |