| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 234,234 | C→T | 78.8% | L183L (TTG→TTA) | gloB ← | predicted hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 234,234 | 0 | C | T | 78.8% | 134.9 / 34.5 | 66 | L183L (TTG→TTA) | gloB | predicted hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (5/9); new base T (26/26); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
TTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA > W3110S.gb/234179‑234297 | ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:1915190/70‑1 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag > 1:126433/1‑70 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:2187767/70‑1 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaaga < 1:2783687/71‑1 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaaga < 1:3368046/71‑1 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaaga < 1:530007/71‑1 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaaga < 1:3083897/71‑1 (MQ=255)ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaaga < 1:989586/71‑1 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:3187028/70‑1 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:2576755/70‑1 (MQ=255) ttGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:369814/66‑1 (MQ=255) gATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:294531/66‑1 (MQ=255) aTTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCa < 1:1908307/71‑1 (MQ=255) tttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCa > 1:3582237/1‑69 (MQ=255) cGTAACTCCTTAACTTTACGATAGTAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACtt > 1:792364/1‑71 (MQ=255) cGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACtt > 1:202098/1‑71 (MQ=255) cGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACtt > 1:1171144/1‑71 (MQ=255) tAACTCCTTAACTTTACGATAATAATCATTTATGGATaaa < 1:2158108/40‑1 (MQ=255) tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCa < 1:1452509/59‑1 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCaaa > 1:77678/1‑59 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCaaa > 1:1910646/1‑59 (MQ=255) cTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAttt > 1:2355499/1‑70 (MQ=255) cTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAttt > 1:1532290/1‑70 (MQ=255) cTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAtt > 1:2728748/1‑69 (MQ=255) aaCTTTACGATAATAATCATTTTTGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGAt > 1:1430565/1‑70 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGATGAATACTCAAAGCAAACTTCATATTTGAt > 1:667623/1‑70 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGAt < 1:2016848/70‑1 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGAt > 1:1563047/1‑70 (MQ=255) aCTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGAt < 1:912522/69‑1 (MQ=255) cTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata < 1:768375/62‑1 (MQ=255) tttACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:2657822/41‑1 (MQ=255) tttACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:1294699/41‑1 (MQ=255) tttACGATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:1194078/41‑1 (MQ=255) tttACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata < 1:992117/61‑1 (MQ=255) tttACGATAATAATCATTTATGGACAAATCGTGCGgaag < 1:411558/39‑1 (MQ=255) tttACCATAATAATCATTTATGGATAAATCGTGCGgaagaa < 1:319333/41‑1 (MQ=38) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa > 1:2586698/1‑41 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa > 1:1556947/1‑41 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa > 1:553978/1‑41 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa > 1:1870746/1‑41 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa > 1:2309245/1‑41 (MQ=255) tACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAgg < 1:758867/69‑1 (MQ=255) aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:2646190/70‑1 (MQ=255) aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:1684970/70‑1 (MQ=255) aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:3020923/70‑1 (MQ=255) taataaTCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt > 1:152560/1‑70 (MQ=255) taataaTCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt > 1:64983/1‑70 (MQ=255) aataatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaaa > 1:3022445/1‑46 (MQ=255) ataatCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:2015729/64‑1 (MQ=255) aatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAgg < 1:1538827/60‑1 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGtata > 1:2731123/1‑63 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGtata > 1:849705/1‑63 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGtata > 1:3337066/1‑63 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGtata > 1:2036828/1‑63 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGtata > 1:2121552/1‑63 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAg < 1:1827450/71‑1 (MQ=255) atCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAg < 1:1207677/71‑1 (MQ=255) aTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcac < 1:2242006/71‑1 (MQ=255) aTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGca > 1:517112/1‑70 (MQ=255) aTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGca > 1:382528/1‑70 (MQ=255) aTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGca > 1:2826895/1‑70 (MQ=255) aTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGca > 1:740717/1‑70 (MQ=255) aTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGca > 1:808640/1‑70 (MQ=255) tATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:1980884/70‑1 (MQ=255) tATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:1479980/70‑1 (MQ=255) tATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa < 1:1475707/70‑1 (MQ=255) | TTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA > W3110S.gb/234179‑234297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |