| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,934,836 | T→C | 11.9% | I70I (ATT→ATC) | dppB → | dipeptide transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,934,836 | 0 | T | C | 11.9% | 105.2 / 7.5 | 42 | I70I (ATT→ATC) | dppB | dipeptide transporter |
| Reads supporting (aligned to +/- strand): ref base T (6/31); new base C (3/2); total (9/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
AGCGTCACGCGCAGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGAAGAGTTCGTGC > W3110S.gb/3934766‑3934906 | agCGTCACGCGCAGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACAtt > 1:3210642/1‑71 (MQ=255) gcgcAGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGt < 1:112103/71‑1 (MQ=255) aGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATg < 1:3708595/71‑1 (MQ=255) aGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATg < 1:2817962/71‑1 (MQ=255) aGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATg < 1:1574916/71‑1 (MQ=255) ctgctgGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCa < 1:660816/71‑1 (MQ=255) gctggctgAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATg > 1:1540959/1‑71 (MQ=255) gctgAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGa < 1:2539117/71‑1 (MQ=255) cGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGt < 1:994408/51‑1 (MQ=255) gCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:2915852/65‑1 (MQ=255) gCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:1113512/65‑1 (MQ=255) gCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:1328576/65‑1 (MQ=255) gCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:1523351/65‑1 (MQ=255) gCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:2540481/65‑1 (MQ=255) gCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:1665074/65‑1 (MQ=255) cTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:1141225/64‑1 (MQ=255) aTAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGt < 1:542931/43‑1 (MQ=255) aTAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGt < 1:1332224/43‑1 (MQ=255) tAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGa < 1:2277408/71‑1 (MQ=255) tAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGa < 1:458172/71‑1 (MQ=255) gcagTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCa < 1:2308634/47‑1 (MQ=255) gcagTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCa < 1:2515056/47‑1 (MQ=255) gcagTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCa < 1:496972/47‑1 (MQ=255) gcagTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCa < 1:3694088/47‑1 (MQ=255) cagTATCTCGATTACATTTGGGGCGTTATGCATGGCGATCTa < 1:3480502/42‑1 (MQ=255) tATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCAt < 1:3000228/62‑1 (MQ=255) tctcCATTACATTTGGGGCGTTATGCATGGCGATc > 1:3707648/1‑35 (MQ=255) tctcCATTACATTTGGGGCGTTAAGCATGGCGATCTAGGCATTTCAATGAAAAGCCg < 1:2602604/57‑1 (MQ=255) ctcCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCg < 1:2184202/56‑1 (MQ=255) ccATTACATCTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCa > 1:3455613/1‑56 (MQ=255) ccATTACATCTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCa > 1:815948/1‑56 (MQ=255) ccATTACATCTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCa > 1:2114441/1‑56 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGTATTTCAATGAAAAGCCGCATCCCGGTTTggg < 1:1209398/67‑1 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAGGCCGCATCCCGGTTTGGGaa > 1:2322010/1‑69 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTggg < 1:41350/67‑1 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTggg < 1:58033/67‑1 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGaa < 1:387320/69‑1 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGaa > 1:2583074/1‑69 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGaa < 1:1495086/69‑1 (MQ=255) cATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGaa > 1:786125/1‑69 (MQ=255) cATTACATCTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTggg < 1:1925740/67‑1 (MQ=255) cATTACATCTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTggg < 1:1936259/67‑1 (MQ=255) aCATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGAAGAGTTc > 1:2756097/1‑71 (MQ=255) ttGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGAAGAGTTCGTGc > 1:1966822/1‑71 (MQ=255) | AGCGTCACGCGCAGCTGCTGGCTGAACTCGGCTTAGATAAACCGATGTGGCAGCAGTATCTCCATTACATTTGGGGCGTTATGCATGGCGATCTAGGCATTTCAATGAAAAGCCGCATCCCGGTTTGGGAAGAGTTCGTGC > W3110S.gb/3934766‑3934906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |