| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,124,493 | T→C | 57.1% | intergenic (+95/‑12) | damX → / → dam | hypothetical protein/DNA adenine methylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,124,493 | 0 | T | C | 57.1% | 5.7 / 21.8 | 21 | intergenic (+95/‑12) | damX/dam | hypothetical protein/DNA adenine methylase |
| Reads supporting (aligned to +/- strand): ref base T (6/3); new base C (5/7); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTTGATGA > W3110S.gb/4124425‑4124563 | tCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcag < 1:1695378/71‑1 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATcagttagt > 1:3348119/1‑71 (MQ=255) gggAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcag < 1:336732/64‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATg > 1:589956/1‑69 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATg > 1:2078155/1‑69 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATg > 1:251922/1‑69 (MQ=255) aGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGaaa < 1:347440/69‑1 (MQ=255) cTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATcgc > 1:381775/1‑71 (MQ=255) gATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCt > 1:1183451/1‑71 (MQ=255) gATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCt > 1:904627/1‑71 (MQ=255) tCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:2504138/70‑1 (MQ=255) tCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGa < 1:2943158/71‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGaa < 1:2073009/70‑1 (MQ=255) cTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTggg < 1:2104974/71‑1 (MQ=255) cTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAg < 1:762684/39‑1 (MQ=255) cTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAg < 1:1749505/39‑1 (MQ=255) cTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAg < 1:1778263/39‑1 (MQ=255) tctcCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAg > 1:2468937/1‑71 (MQ=255) tctcCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAg > 1:2583006/1‑71 (MQ=255) tctcCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAg > 1:3595027/1‑71 (MQ=255) tctcCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAg > 1:2106133/1‑71 (MQ=255) agaAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATcc < 1:1104139/71‑1 (MQ=255) tAATCAGTTAGTCAGCATGAAGAAAAATCGCGCttttt > 1:2380679/1‑38 (MQ=255) tAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTg < 1:2143592/45‑1 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGa < 1:2956028/38‑1 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGa < 1:2891856/38‑1 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGa < 1:446754/38‑1 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGa < 1:2501129/38‑1 (MQ=255) tagttagtCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTtgatga > 1:3202488/1‑71 (MQ=255) tagttagtCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTtgatga > 1:2730475/1‑71 (MQ=255) tagttagtCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTtgatga > 1:2726429/1‑71 (MQ=255) | TCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTTGATGA > W3110S.gb/4124425‑4124563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |