| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,154,666 | A→G | 100% | I23V (ATT→GTT) | yhfA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,154,666 | 0 | A | G | 100.0% | 163.7 / NA | 46 | I23V (ATT→GTT) | yhfA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/31); total (15/31) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAAATGCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTT > W3110S.gb/4154597‑4154721 | aaaaTGCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGa < 1:1925670/70‑1 (MQ=255) aaTGCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGttt > 1:3636491/1‑71 (MQ=255) aTGCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:2536468/71‑1 (MQ=255) aTGCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:3386074/71‑1 (MQ=255) gAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAg > 1:93305/1‑64 (MQ=255) gAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAg > 1:2356978/1‑64 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:839841/1‑70 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:161194/1‑70 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:3523917/1‑70 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:3456856/1‑70 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:1354152/1‑70 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:2189749/1‑68 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCAc > 1:796479/1‑68 (MQ=255) cTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:3305704/41‑1 (MQ=255) cTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:3128005/41‑1 (MQ=255) cTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:3125703/41‑1 (MQ=255) cTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:3074659/41‑1 (MQ=255) cTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:3062002/41‑1 (MQ=255) cTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:1823636/41‑1 (MQ=255) ttCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTcc < 1:1550856/70‑1 (MQ=255) ttCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTcc < 1:3144892/70‑1 (MQ=255) ttCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTcc < 1:3217202/70‑1 (MQ=255) ggCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGa < 1:3723972/71‑1 (MQ=255) gCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCTATGGaa < 1:475366/71‑1 (MQ=255) cGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATgg > 1:3654885/1‑68 (MQ=255) cGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGaaa > 1:783978/1‑71 (MQ=255) tCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGgt < 1:927399/71‑1 (MQ=255) tCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGgt < 1:2923752/71‑1 (MQ=255) tCCGCCACTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGgt < 1:3010318/71‑1 (MQ=255) cGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGgtgt > 1:1390089/1‑71 (MQ=255) cGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGgtgt > 1:1557614/1‑71 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2191409/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:680527/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2461547/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:436160/71‑1 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt > 1:3174511/1‑71 (MQ=255) gCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:3200208/71‑1 (MQ=255) ctctGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2723637/69‑1 (MQ=255) tcatcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:4423/63‑1 (MQ=255) tcatcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:3216291/63‑1 (MQ=255) tcatcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:532748/63‑1 (MQ=255) tcatcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2049194/63‑1 (MQ=255) tcatcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:1404042/63‑1 (MQ=255) tcatcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2947289/63‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCTATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2375014/60‑1 (MQ=255) tcaGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2732804/60‑1 (MQ=255) caGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:442291/59‑1 (MQ=255) | AAAATGCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTT > W3110S.gb/4154597‑4154721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |