| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,177,469 | G→A | 100% | A9V (GCG→GTG) | gspK ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,177,469 | 0 | G | A | 85.0% | 95.3 / 10.9 | 40 | A9V (GCG→GTG) | gspK | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base A (22/12); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGTCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGACTCT > W3110S.gb/4177415‑4177532 | aTGTCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCgttgtt < 1:2644954/70‑1 (MQ=255) tCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCat < 1:2697740/70‑1 (MQ=255) tCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCat < 1:1825800/70‑1 (MQ=255) ctgcCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt < 1:1171641/70‑1 (MQ=255) ctgcCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt < 1:3298341/70‑1 (MQ=255) gcCATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTtattat < 1:845982/71‑1 (MQ=255) ccATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt < 1:1001653/58‑1 (MQ=255) ccATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt < 1:262659/58‑1 (MQ=255) ccATGAGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCattatt < 1:1329343/58‑1 (MQ=255) aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat > 1:2780493/1‑62 (MQ=255) aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat > 1:2708545/1‑62 (MQ=255) aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat > 1:3647239/1‑62 (MQ=255) aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat > 1:1249790/1‑62 (MQ=255) aGCGCCAGTAACATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGttat > 1:2364125/1‑62 (MQ=255) gTAACATGAGTACAATGAGCAGCACGACGCCGCgttgtt < 1:2272637/39‑1 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt > 1:3599384/1‑37 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt > 1:879277/1‑37 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt > 1:1068183/1‑37 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCgttgtt > 1:1743801/1‑37 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt > 1:2480123/1‑71 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt > 1:214628/1‑71 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt > 1:1965189/1‑71 (MQ=255) aaCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCt > 1:1162461/1‑71 (MQ=255) ctgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg < 1:2315897/68‑1 (MQ=255) ctgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg < 1:1298804/68‑1 (MQ=255) ctgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg < 1:2974220/68‑1 (MQ=255) aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa > 1:1357602/1‑38 (MQ=255) aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa > 1:1270198/1‑38 (MQ=255) aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa > 1:3318116/1‑38 (MQ=255) aCATGAGTACAATGAGCAGCACGACGCCGCGTTGTTCa > 1:3413629/1‑38 (MQ=255) tgtGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTg < 1:3090465/68‑1 (MQ=255) gAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCt > 1:1349522/1‑71 (MQ=255) aGTACAATGAGCAGCACGACGCCGCGTTGTTCatta > 1:1289343/1‑36 (MQ=255) tACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGc < 1:2830/71‑1 (MQ=255) tACAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGGCTGAGCTGGc < 1:192607/71‑1 (MQ=255) cAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGa < 1:327281/71‑1 (MQ=255) cAATGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGa > 1:3625495/1‑71 (MQ=255) tGAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGActc > 1:2367404/1‑71 (MQ=255) gAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGActct > 1:3022665/1‑71 (MQ=255) gAGCAGCACGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGActct > 1:2703765/1‑71 (MQ=255) | ATGTCTGCTGCCAGAGCAGCCATGAGCGCCAGTAACATGAGTACAATGAGCAGCGCGACGCCGCGTTGTTCATTATTCATGGTTATTATTCCCGGCCTGCGTCTGAGCTGGCGACTCT > W3110S.gb/4177415‑4177532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |