| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,246,866 | T→C | 31.4% | V81V (GTA→GTG) | malG ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,246,866 | 0 | T | C | 31.4% | 41.6 / 28.1 | 35 | V81V (GTA→GTG) | malG | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (16/8); new base C (2/9); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGC > W3110S.gb/4246806‑4246925 | cgcCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATg > 1:503995/1‑71 (MQ=255)cgcCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATg > 1:3068927/1‑71 (MQ=255) aTGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTgg > 1:956366/1‑71 (MQ=255) aTGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTgg > 1:3540583/1‑71 (MQ=255) aTGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTg > 1:2154186/1‑70 (MQ=255) ccAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGt < 1:963418/71‑1 (MQ=255) ccAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGt < 1:133735/71‑1 (MQ=255) ccAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:981021/71‑1 (MQ=255) ccAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:3625228/71‑1 (MQ=255) cAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:59275/70‑1 (MQ=255) cgcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:518651/1‑68 (MQ=255) cgcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:2946116/1‑68 (MQ=255) gcgGAAATCCCGGGGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:2792957/1‑67 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:988804/1‑67 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:3208333/1‑67 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:29138/1‑67 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTaa > 1:2675657/1‑67 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATg > 1:3694845/1‑55 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGc < 1:707298/70‑1 (MQ=255) ccGGCGACCTTTACC‑AGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGcc < 1:1268769/71‑1 (MQ=255) ccGGCGACCTTTACC‑AGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGcc < 1:1272959/71‑1 (MQ=255) cGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGt < 1:3687288/55‑1 (MQ=255) cGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGcc < 1:3073960/71‑1 (MQ=255) cGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGcc < 1:1641760/71‑1 (MQ=255) tACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCaa > 1:1089339/1‑68 (MQ=255) tACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCaa > 1:230362/1‑68 (MQ=255) tACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCaa > 1:240755/1‑68 (MQ=255) tACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCaa > 1:265486/1‑68 (MQ=255) ccacagccacagCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:90267/70‑1 (MQ=255) ccacagccacagCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:135844/70‑1 (MQ=255) ccacagccacagCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:2736496/70‑1 (MQ=255) ccacagccacagCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:985881/70‑1 (MQ=255) ccacagccacagCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:2902493/70‑1 (MQ=255) ccacagccacagCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:2250917/70‑1 (MQ=255) gccacagcAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGc > 1:2367612/1‑70 (MQ=255) tACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGtcc < 1:393415/42‑3 (MQ=255) tACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTc < 1:3164643/42‑1 (MQ=255) tACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTaaa < 1:3662169/51‑1 (MQ=255) tACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTaaa < 1:1375082/51‑1 (MQ=255) | CGCCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGC > W3110S.gb/4246806‑4246925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |