Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,295,565 T→C 100% F154F (TTT→TTC nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,295,5650TC89.4% 195.9 / 11.6 66F154F (TTT→TTCnrfEheme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
Reads supporting (aligned to +/- strand):  ref base T (6/1);  new base C (28/31);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAG  >  W3110S.gb/4295497‑4295630
                                                                    |                                                                 
cGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 <  1:3454159/71‑1 (MQ=255)
cGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 <  1:152387/71‑1 (MQ=255)
cGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 <  1:2537396/71‑1 (MQ=255)
cGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 <  1:609123/71‑1 (MQ=255)
     atTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCAc                                                            >  1:3491032/1‑71 (MQ=255)
     atTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCAc                                                            >  1:1917023/1‑71 (MQ=255)
          caccaGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCgctg                                                       <  1:2242055/71‑1 (MQ=255)
              aGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCttt                                                   <  1:2506466/71‑1 (MQ=255)
              aGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCttt                                                   <  1:286168/71‑1 (MQ=255)
                    cGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTg                                             >  1:2277482/1‑71 (MQ=255)
                    cGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTg                                             >  1:1886064/1‑71 (MQ=255)
                    cGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTg                                             >  1:1786806/1‑71 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:2306750/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:3063953/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:2063670/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:1047626/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:2948639/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:1825210/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:2809856/71‑1 (MQ=255)
                      aaGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:2469288/71‑1 (MQ=255)
                       aGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGc                                           <  1:173467/70‑1 (MQ=255)
                       aGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCt                                          <  1:2957734/71‑1 (MQ=255)
                            gcgATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 >  1:2390184/1‑43 (MQ=255)
                            gcgATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 >  1:1479234/1‑43 (MQ=255)
                            gcgATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCa                                                                 >  1:2839369/1‑43 (MQ=255)
                              gATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCgg                                   >  1:1578852/1‑71 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCgctg                                                       >  1:2111759/1‑43 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:3694471/1‑71 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:40951/1‑71 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:2352128/1‑71 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:2917517/1‑71 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:2588347/1‑71 (MQ=255)
                                      tCCGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:1623189/1‑71 (MQ=255)
                                       ccGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATgg                           >  1:2862047/1‑70 (MQ=255)
                                        cGATGCTGCAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGt                          <  1:23803/70‑1 (MQ=255)
                                           tgctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAgcg                      >  1:2728575/1‑71 (MQ=255)
                                            gctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTa                                         >  1:1227378/1‑51 (MQ=255)
                                            gctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTa                                         >  1:1264162/1‑51 (MQ=255)
                                            gctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTa                                         >  1:550114/1‑51 (MQ=255)
                                             ctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAgcggc                    <  1:1443787/71‑1 (MQ=255)
                                             ctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAgcggc                    <  1:1257098/71‑1 (MQ=255)
                                             ctgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAgcggc                    <  1:2400740/71‑1 (MQ=255)
                                              tgcAACATCCCGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAgcggc                    <  1:2452944/70‑1 (MQ=255)
                                                     tCCCGGTCTTATCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTgg            >  1:2457302/1‑71 (MQ=255)
                                                     tCCCGGTCTTATCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTgg            <  1:1771106/71‑1 (MQ=255)
                                                      cccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCt                                          <  1:2734476/40‑1 (MQ=255)
                                                       ccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCg               >  1:1068845/1‑66 (MQ=255)
                                                       ccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCg               >  1:44249/1‑66 (MQ=255)
                                                       ccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCg               >  1:2040979/1‑66 (MQ=255)
                                                       ccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTgg            <  1:3023191/69‑1 (MQ=255)
                                                       ccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGgcg          >  1:122317/1‑71 (MQ=255)
                                                       ccGGTCTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCAGCGAGCGTgg            <  1:1732862/69‑1 (MQ=255)
                                                            cTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAgcggc                    >  1:2642730/1‑56 (MQ=255)
                                                            cTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGc     >  1:3311882/1‑71 (MQ=255)
                                                            cTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGc     <  1:972376/71‑1 (MQ=255)
                                                            cTTATCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGAGGCGCTGGc     >  1:166641/1‑71 (MQ=255)
                                                               aTCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  >  1:2294164/1‑71 (MQ=255)
                                                               aTCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  >  1:1910059/1‑71 (MQ=255)
                                                               aTCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  >  1:1476568/1‑71 (MQ=255)
                                                               aTCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  >  1:735735/1‑71 (MQ=255)
                                                               aTCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  >  1:1204203/1‑71 (MQ=255)
                                                                tCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  <  1:2372083/70‑1 (MQ=255)
                                                                tCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  <  1:2094436/70‑1 (MQ=255)
                                                                tCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  <  1:1863258/70‑1 (MQ=255)
                                                                tCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  <  1:1390195/70‑1 (MQ=255)
                                                                tCTTCCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAg  <  1:888777/70‑1 (MQ=255)
                                                                    |                                                                 
CGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAG  >  W3110S.gb/4295497‑4295630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: