| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 45,813 | C→T | 100% | P3S (CCG→TCG) | yaaU → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 45,813 | 0 | C | T | 89.2% | 96.1 / 4.6 | 37 | P3S (CCG→TCG) | yaaU | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (18/15); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGC > W3110S.gb/45748‑45878 | tGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTc > 1:1371495/1‑70 (MQ=255) tttGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACttt < 1:2429980/71‑1 (MQ=255) tACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGa > 1:3404325/1‑71 (MQ=255) tACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGa > 1:2966664/1‑71 (MQ=255) aCTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTctcc > 1:1119260/1‑71 (MQ=255) aCTGACTCTCATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTctcc > 1:2908063/1‑71 (MQ=255) aCTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTctcctc < 1:548958/69‑1 (MQ=255) tcATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTAtt < 1:788542/69‑1 (MQ=255) tcATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTAtt < 1:3456883/69‑1 (MQ=255) tcATTGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTAtt < 1:338264/69‑1 (MQ=255) aTTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:2678875/68‑1 (MQ=255) aTTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:2649885/68‑1 (MQ=255) ttGCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCa < 1:527915/52‑1 (MQ=255) gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGAc > 1:1433577/1‑43 (MQ=255) gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCAccgcc > 1:901414/1‑71 (MQ=255) gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCAccgcc > 1:274505/1‑71 (MQ=255) gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCAccgcc > 1:1986016/1‑71 (MQ=255) gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCAccgcc > 1:1070718/1‑71 (MQ=255) gCAAAAAACAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCAccgcc > 1:1438882/1‑71 (MQ=255) cAGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTAtt < 1:1480250/56‑1 (MQ=255) aGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGc < 1:853548/71‑1 (MQ=255) aGGAATAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGc < 1:2895423/71‑1 (MQ=255) aaTAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCtgt < 1:496115/71‑1 (MQ=255) aTAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCtgt < 1:745539/70‑1 (MQ=255) aTAACAATGCAATAGTCCAGAAAATTTGACGATCTCAAATTATCCTCTa > 1:1407605/1‑49 (MQ=25) tAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTgg > 1:810732/1‑71 (MQ=255) tAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTgg > 1:2698076/1‑71 (MQ=255) tAACCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTgg > 1:1239448/1‑71 (MQ=255) aCCATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTggg > 1:102871/1‑70 (MQ=255) aCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTggg > 1:2014580/1‑70 (MQ=255) ccATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:37334/48‑1 (MQ=255) ccATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:3643671/48‑1 (MQ=255) ccATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:1501016/48‑1 (MQ=255) ccATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:1689032/48‑1 (MQ=255) ccATGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTc < 1:2606409/48‑1 (MQ=255) tGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGc > 1:1085144/1‑71 (MQ=255) tGCAATCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGc > 1:2163553/1‑71 (MQ=255) | TGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGC > W3110S.gb/45748‑45878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |