| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,486,264 | C→T | 100% | P752P (CCG→CCA) | valS ← | valyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,486,264 | 0 | C | T | 98.1% | 151.5 / ‑4.1 | 51 | P752P (CCG→CCA) | valS | valyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (21/29); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTC > W3110S.gb/4486201‑4486329 | ggCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa > 1:104697/1‑67 (MQ=255)ggCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATgcg > 1:1873739/1‑71 (MQ=255)ggCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGAGATGAACGGAATGATTGGATgcg > 1:534009/1‑71 (MQ=255) gATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgc > 1:22621/1‑71 (MQ=255) gATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgc > 1:2593164/1‑71 (MQ=255) gATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgc > 1:3165836/1‑71 (MQ=255) aTACCGCAAAGTAGTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgcg > 1:2810070/1‑71 (MQ=255) aTACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgc < 1:3162822/70‑1 (MQ=255) aTACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgc < 1:1910322/70‑1 (MQ=255) aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGa < 1:3089636/71‑1 (MQ=255) cTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCgcag < 1:356954/61‑1 (MQ=255) cTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCgcag < 1:2610531/61‑1 (MQ=255) cTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCgcag < 1:146576/61‑1 (MQ=255) tCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg < 1:2265398/70‑1 (MQ=255) cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg < 1:3547863/40‑1 (MQ=255) cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg < 1:3704224/40‑1 (MQ=255) cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg < 1:2594707/40‑1 (MQ=255) cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg < 1:1852227/40‑1 (MQ=255) cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg < 1:1685416/40‑1 (MQ=255) cacGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg < 1:1801159/40‑1 (MQ=255) gCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTc < 1:252603/71‑1 (MQ=255) gCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCAc > 1:2195665/1‑70 (MQ=255) gCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCAc > 1:3648841/1‑70 (MQ=255) gCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCAc > 1:1875391/1‑70 (MQ=255) aGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGc > 1:1724687/1‑71 (MQ=255) gATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:980699/71‑1 (MQ=255) aTGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGct < 1:355291/70‑2 (MQ=255) aTGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:138074/70‑1 (MQ=255) aTGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:1955777/70‑1 (MQ=255) tGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTa > 1:345761/1‑71 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTAt > 1:2884258/1‑71 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTAt > 1:1830957/1‑71 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTAt > 1:100799/1‑71 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTAt > 1:1920066/1‑71 (MQ=255) tttCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg < 1:682971/52‑1 (MQ=255) tCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGc > 1:601171/1‑70 (MQ=255) tCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGc > 1:733770/1‑70 (MQ=255) ggTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg < 1:724019/48‑1 (MQ=255) ggTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAg < 1:3361594/71‑1 (MQ=255) ggTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCg < 1:1133810/69‑1 (MQ=255) cGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCAcc < 1:3590214/49‑1 (MQ=255) cGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCAcc < 1:2161307/49‑1 (MQ=255) cGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCAcc < 1:385785/49‑1 (MQ=255) ggAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAg < 1:1834987/71‑1 (MQ=255) ggAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAg < 1:3315292/71‑1 (MQ=255) gAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAg > 1:1663172/1‑49 (MQ=255) aTGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTc > 1:2016405/1‑71 (MQ=255) aTGATTAGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTc > 1:60002/1‑71 (MQ=255) tGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:692598/48‑1 (MQ=255) tGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:734766/48‑1 (MQ=255) tGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:1701536/48‑1 (MQ=255) | GGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGTTC > W3110S.gb/4486201‑4486329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |