| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,547,549 | T→C | 13.8% | intergenic (+234/‑248) | fimE → / → fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,547,549 | 0 | T | C | 13.8% | 166.0 / 17.4 | 65 | intergenic (+234/‑248) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin |
| Reads supporting (aligned to +/- strand): ref base T (22/34); new base C (3/6); total (25/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTA > W3110S.gb/4547482‑4547612 | taattTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATtcttct > 1:3610644/1‑71 (MQ=255)taattTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATtcttct > 1:1952214/1‑71 (MQ=255)taattTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATtcttct > 1:2137893/1‑71 (MQ=255) attTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctc < 1:3305687/70‑1 (MQ=255) attTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctc < 1:2719723/70‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:973367/71‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:174050/71‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:1892105/71‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:1583510/71‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:1487704/71‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:2759163/71‑1 (MQ=255) acaTCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa < 1:1319240/71‑1 (MQ=255) aCCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCTACGCATCTTCCTCATTCTTCTCTCCAAAAacca > 1:1608144/1‑71 (MQ=255) aCCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAacca > 1:794515/1‑71 (MQ=255) ccTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaa < 1:2108612/64‑1 (MQ=255) ccTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaa < 1:247824/64‑1 (MQ=255) ccTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaa < 1:3213609/64‑1 (MQ=255) ccTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaa < 1:1250305/64‑1 (MQ=255) ccTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaa < 1:1883710/64‑1 (MQ=255) atatGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGc > 1:2524112/1‑70 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctct > 1:3657009/1‑49 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctct > 1:1695558/1‑49 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAata > 1:2874090/1‑71 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAata > 1:2173334/1‑71 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTACAAAAACCACCTCATGCAata > 1:3685473/1‑71 (MQ=255) tAAAGCTAACGTTTCTGTGGCTCGACGCAGCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAAtata < 1:594571/71‑1 (MQ=255) aaGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATaaa < 1:1401261/71‑1 (MQ=255) gTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCtata < 1:1870635/71‑1 (MQ=255) tttCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCaa < 1:1985796/56‑1 (MQ=255) tttCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCaa < 1:412832/56‑1 (MQ=255) tttCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCaa < 1:846894/56‑1 (MQ=255) tttCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCaa < 1:2742275/56‑1 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCta > 1:2893623/1‑66 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaa > 1:2478430/1‑70 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaa > 1:46294/1‑70 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaa > 1:2813643/1‑70 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaa > 1:3307913/1‑70 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaa > 1:1713570/1‑70 (MQ=255) tCTGTGGCTAGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaa > 1:1827306/1‑70 (MQ=255) tgtgGCTCGACGCATCTTCCTCATTCCTCTCTCCaa < 1:1989142/36‑1 (MQ=255) gCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:2603701/71‑1 (MQ=255) gCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:675015/71‑1 (MQ=255) gCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:1546142/71‑1 (MQ=255) gCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:2039538/71‑1 (MQ=255) gCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:2439166/71‑1 (MQ=255) gCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:2731547/71‑1 (MQ=255) tCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATaa > 1:1051529/1‑71 (MQ=255) aCGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACaa < 1:532418/71‑1 (MQ=255) aCGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACaa < 1:2122957/71‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:1916746/61‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:640505/61‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:609385/61‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:1793774/69‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:1353838/69‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:2536285/69‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:3044425/69‑1 (MQ=255) tCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:1828601/69‑1 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCaa > 1:426058/1‑36 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATaa > 1:1366218/1‑61 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATaa > 1:700170/1‑61 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATaa > 1:1046737/1‑61 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAata > 1:913178/1‑71 (MQ=255) ttCCTCATTCCTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:157602/1‑69 (MQ=255) ttCCTCATTCCTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:296823/1‑69 (MQ=255) ttCCTCATTCCTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:1139507/1‑69 (MQ=255) ccTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa < 1:2513692/67‑1 (MQ=255) tCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:618078/63‑1 (MQ=255) cATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTa > 1:1702774/1‑69 (MQ=255) | TAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTA > W3110S.gb/4547482‑4547612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |