| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,549,768 | C→T | 75.9% | intergenic (+57/‑10) | fimC → / → fimD | chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,549,768 | 0 | C | T | 75.9% | 49.4 / 14.2 | 29 | intergenic (+57/‑10) | fimC/fimD | chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base T (9/13); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGC > W3110S.gb/4549706‑4549822 | gAATAACGCAGGGGTAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaat < 1:1876607/71‑1 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTggag > 1:1595558/1‑67 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTggag > 1:2360394/1‑67 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaat < 1:749866/71‑1 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaat < 1:741116/71‑1 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaat < 1:3720312/71‑1 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaat < 1:2097263/71‑1 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaa > 1:2741962/1‑70 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGCAGGaat < 1:479106/71‑1 (MQ=255)gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGaat < 1:381594/71‑1 (MQ=255) aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGt < 1:574596/71‑1 (MQ=255) aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGt > 1:1369279/1‑71 (MQ=255) taatGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGAc < 1:1033287/68‑1 (MQ=255) aaTAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTaa < 1:2290287/63‑1 (MQ=255) aaTAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc > 1:2646627/1‑71 (MQ=255) tAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc > 1:170180/1‑69 (MQ=255) aaaGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaat < 1:3126072/38‑1 (MQ=255) aaGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa < 1:2914257/71‑1 (MQ=255) gACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTAc > 1:1715062/1‑58 (MQ=255) tGCTGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAcc < 1:243097/56‑1 (MQ=255) tGCTGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAcc < 1:1476941/56‑1 (MQ=255) tGCCGGGTGATTTTAAGCTGGAGGAATAATGTCat > 1:1046327/1‑35 (MQ=255) tGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAAt > 1:3524948/1‑71 (MQ=255) ccGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGc < 1:376774/71‑1 (MQ=255) ccGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGc < 1:1334378/71‑1 (MQ=255) ttttAAGCTGGAGGAATAATGTCATATCTGAATTTaa < 1:2789767/37‑1 (MQ=255) ttttAAGCTGGAGGAATAATGTCATATCTGAATTTaa < 1:1334513/37‑1 (MQ=255) ttAAGCTGGAGGAATAATGTCATATCTGAATTTaa > 1:3517024/1‑35 (MQ=255) ttAAGCTGGAGGAATAATGTCATATCTGAATTTaa > 1:3521405/1‑35 (MQ=255) | GAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGC > W3110S.gb/4549706‑4549822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |