Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	108.4 / NA	36	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/18);  total (18/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG  >  W3110S.gb/4563532‑4563654                                                          |                                                                  agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                      >  1:1551565/1‑71 (MQ=255)                acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                       <  1:427723/71‑1 (MQ=255)                acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                       <  1:2986261/71‑1 (MQ=255)                     gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc                                  >  1:291750/1‑71 (MQ=255)                     gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc                                  >  1:2320779/1‑71 (MQ=255)                     gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc                                  >  1:2208382/1‑71 (MQ=255)                          tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACg                                   >  1:1451408/1‑65 (MQ=255)                           ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                        >  1:3164331/1‑59 (MQ=255)                           ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                        >  1:2949941/1‑59 (MQ=255)                           ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                        >  1:1816588/1‑59 (MQ=255)                           ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                        >  1:2012323/1‑59 (MQ=255)                           ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                        >  1:2410384/1‑59 (MQ=255)                             ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                           <  1:3709163/70‑1 (MQ=255)                             ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                           <  1:1791049/70‑1 (MQ=255)                                    gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                       <  1:1321992/51‑1 (MQ=255)                                    gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                       <  1:651816/51‑1 (MQ=255)                                      cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg                  >  1:97972/1‑70 (MQ=255)                                           aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc            <  1:3515600/71‑1 (MQ=255)                                           aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc            <  1:2991480/71‑1 (MQ=255)                                              ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc         <  1:2064900/71‑1 (MQ=255)                                              ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc         <  1:1340078/71‑1 (MQ=255)                                                   tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                     <  1:3493631/54‑1 (MQ=255)                                                   tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                     <  1:633228/54‑1 (MQ=255)                                                   tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc                     <  1:2152698/54‑1 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt                             >  1:2856798/1‑44 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt                             >  1:1540901/1‑44 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                   >  1:2912575/1‑54 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                   >  1:1664640/1‑54 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                   >  1:2629381/1‑54 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                   >  1:2015783/1‑54 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa                   >  1:1248474/1‑54 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg  <  1:1696614/71‑1 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg  <  1:1588823/71‑1 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg  <  1:3085206/71‑1 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg  <  1:1490211/71‑1 (MQ=255)                                                     aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg  <  1:394938/71‑1 (MQ=255)                                                          |                                                                  AGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG  >  W3110S.gb/4563532‑4563654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑