| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 249,599 | A→G | 31.0% | pseudogene (472/1713 nt) | fhiA ← | ECK0230:JW5811:b0229; flagellar system protein, promoterless fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 249,599 | 0 | A | G | 31.0% | 33.4 / 21.3 | 29 | pseudogene (472/1713 nt) | fhiA | ECK0230:JW5811:b0229; flagellar system protein, promoterless fragment |
| Reads supporting (aligned to +/- strand): ref base A (9/11); new base G (5/4); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGA > W3110S.gb/249533‑249667 | gCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACggg < 1:279353/71‑1 (MQ=255)gCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACggg < 1:2714930/71‑1 (MQ=255)gCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACggg < 1:2580627/71‑1 (MQ=255) ttCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCcagc < 1:969166/68‑1 (MQ=255) tCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCcagc < 1:2079487/67‑1 (MQ=255) cccGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGGCGGGCTTGCCAGCAGCTg > 1:2898907/1‑71 (MQ=255) cccGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTg > 1:1520407/1‑71 (MQ=255) cGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGt < 1:3198660/71‑1 (MQ=255) aCGAACATAATCCCGGTGGCGGTGTAGAGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGc < 1:2034045/71‑1 (MQ=255) aCGAACATAATCCCGGTGGCGGTGTAGAGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGc < 1:2747834/71‑1 (MQ=255) aaCATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGat < 1:1842429/71‑1 (MQ=255) aaCATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGat < 1:1035689/71‑1 (MQ=255) tAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACg < 1:3561105/52‑1 (MQ=255) tAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACg < 1:2080104/52‑1 (MQ=255) tAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACg < 1:2826042/52‑1 (MQ=255) gtAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgc > 1:3166509/1‑71 (MQ=255) gtAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgc > 1:1743226/1‑71 (MQ=255) gtAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgc > 1:599541/1‑71 (MQ=255) gtAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgc > 1:310849/1‑71 (MQ=255) gtAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgc > 1:2281241/1‑71 (MQ=255) gtAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCAGTTGTCGCTGAcg > 1:351986/1‑70 (MQ=255) tAGAGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgcg < 1:115170/71‑1 (MQ=255) tAGAGCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGAcgcg < 1:3327929/71‑1 (MQ=255) gagCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCg > 1:3655902/1‑61 (MQ=255) gagCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCggg > 1:2524094/1‑71 (MQ=255) gagCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCggg > 1:1244124/1‑71 (MQ=255) gagCACCGGCGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCggg > 1:697477/1‑71 (MQ=255) cACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGa > 1:2118912/1‑71 (MQ=255) gACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGa > 1:2154259/1‑70 (MQ=255) | GCAGGTGCGGCATTCCCGGTACCACCGCCAGCACGAACATAATCCCGGTGGCGGTGTAGAGCACCGACGGGCTTGCCAGCAGCTGGTTACGCACGTCATGGGCGATATCGCCGTTGTCGCTGACGCGGGTGACGA > W3110S.gb/249533‑249667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |