Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,629,902 C→T 100% T109M (ACG→ATG)  serB → 3‑phosphoserine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,629,9020CT96.3% 75.6 / ‑3.8 27T109M (ACG→ATG) serB3‑phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (11/15);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTG  >  W3110S.gb/4629842‑4629967
                                                            |                                                                 
gCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTg                                                         <  1:2135132/71‑1 (MQ=255)
gCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTg                                                         <  1:1422977/71‑1 (MQ=255)
gCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTg                                                         <  1:1505969/71‑1 (MQ=255)
     gCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGt                                                    <  1:254884/71‑1 (MQ=255)
     gCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGt                                                    <  1:818584/71‑1 (MQ=255)
     gCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGt                                                    <  1:2243475/71‑1 (MQ=255)
           gAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGa                                              <  1:1920427/71‑1 (MQ=255)
                     tGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTGCt                                                       >  1:3467068/1‑52 (MQ=255)
                           tCGCCCCCCTGGGGAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGc                               <  1:3351842/70‑1 (MQ=255)
                                     ggggAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGAtt                     >  1:3431128/1‑70 (MQ=255)
                                      gggAAAATCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGa                   <  1:1433976/71‑1 (MQ=255)
                                             tCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTAtt            <  1:2494860/71‑1 (MQ=255)
                                             tCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTAtt            <  1:1012983/71‑1 (MQ=255)
                                             tCCCGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTAtt            <  1:3648857/71‑1 (MQ=255)
                                               ccGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtga          >  1:820190/1‑71 (MQ=255)
                                               ccGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtga          >  1:1311087/1‑71 (MQ=255)
                                               ccGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtga          >  1:1162137/1‑71 (MQ=255)
                                               ccGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtga          >  1:35684/1‑71 (MQ=255)
                                               ccGCACCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtga          >  1:3322658/1‑71 (MQ=255)
                                               ccGCACCTACGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtga          >  1:379528/1‑71 (MQ=255)
                                                  cACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATtgatga       <  1:1050169/71‑1 (MQ=255)
                                                   aCCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGaa      <  1:3441026/71‑1 (MQ=255)
                                                   aCCTGCGCATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGaa      <  1:1357400/71‑1 (MQ=255)
                                                     cTGCGCATGCCGTGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGaa      <  1:2544994/69‑1 (MQ=255)
                                                       gcgcATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTg  >  1:28590/1‑71 (MQ=255)
                                                       gcgcATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTg  >  1:2740607/1‑71 (MQ=255)
                                                       gcgcATGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTg  >  1:2462579/1‑71 (MQ=255)
                                                            |                                                                 
GCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTG  >  W3110S.gb/4629842‑4629967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑