| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,640,981 | C→T | 30.0% | A98V (GCT→GTT) | creB → | DNA‑binding response regulator in two‑component regulatory system with CreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,640,981 | 0 | C | T | 30.0% | 43.2 / 19.3 | 30 | A98V (GCT→GTT) | creB | DNA‑binding response regulator in two‑component regulatory system with CreC |
| Reads supporting (aligned to +/- strand): ref base C (14/7); new base T (5/4); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCG > W3110S.gb/4640916‑4641047 | aCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgac > 1:1870899/1‑70 (MQ=255)aCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgac > 1:3426818/1‑70 (MQ=255)aCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgac > 1:3284961/1‑70 (MQ=255) ttacTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGc > 1:2994674/4‑71 (MQ=255) ttCTTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGc > 1:3219820/1‑71 (MQ=255) ttCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGc > 1:1096937/1‑71 (MQ=255) ttCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGc > 1:3542796/1‑71 (MQ=255) ttCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGc > 1:2788785/1‑71 (MQ=255) cGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:1229226/71‑1 (MQ=255) aaGAGGGCGATCGCCTGCTTGGGCTGGAAATTGGGGTTGACGACTACGTGGCTaa < 1:369378/55‑1 (MQ=37) gagGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGa < 1:1700125/71‑1 (MQ=255) cGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAgt > 1:721428/1‑69 (MQ=255) cGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAgt > 1:1714610/1‑69 (MQ=255) cGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAgt > 1:1942158/1‑69 (MQ=255) cGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcgc > 1:1111815/1‑71 (MQ=255) cGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcgc > 1:3197208/1‑71 (MQ=255) cGCCTGCTTGGGCTGGAAATTGATGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTgcgc > 1:3456434/1‑71 (MQ=255) ccTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc < 1:2378424/70‑1 (MQ=255) ccTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc < 1:756971/70‑1 (MQ=255) ccTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc < 1:939773/70‑1 (MQ=255) ggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:2418152/38‑1 (MQ=255) ggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCg < 1:1709306/38‑1 (MQ=255) ggCGGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGGgcgc < 1:3696425/71‑1 (MQ=255) gCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAgtg > 1:3590656/1‑55 (MQ=255) gCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCGAGGGTGCGCa > 1:1159185/1‑71 (MQ=255) gAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCt < 1:3098025/70‑1 (MQ=255) gAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCt < 1:3716444/70‑1 (MQ=255) ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTAc > 1:3540045/1‑67 (MQ=255) ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGc > 1:1547753/1‑70 (MQ=255) ggTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCg > 1:573920/1‑71 (MQ=255) | ACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCG > W3110S.gb/4640916‑4641047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |