| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 344,381 | G→A | 100% | intergenic (+166/‑247) | yahL → / → yahM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 344,381 | 0 | G | A | 94.4% | 150.2 / 1.0 | 53 | intergenic (+166/‑247) | yahL/yahM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (30/20); total (33/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAT > W3110S.gb/344314‑344448 | ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:3531827/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:2050509/1‑71 (MQ=255)ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt > 1:1583224/1‑71 (MQ=255) cATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt < 1:1889255/70‑1 (MQ=255) cATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt < 1:3088437/70‑1 (MQ=255) cATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGAttt < 1:536142/70‑1 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2737029/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:2721928/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAg > 1:1185780/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAg > 1:491988/1‑71 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAg > 1:1204740/1‑71 (MQ=255) ttttCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCAt > 1:2874927/1‑71 (MQ=255) ttttCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCAt > 1:1733899/1‑71 (MQ=255) tCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGc < 1:3556457/70‑1 (MQ=255) tCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGc < 1:2807673/70‑1 (MQ=255) gCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCATTAGTGATTTCGCAGCCATGCgg < 1:1758145/70‑1 (MQ=255) ttgttgTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaa < 1:3070586/70‑1 (MQ=255) ttgttgTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaa > 1:1473774/1‑70 (MQ=255) ttgttgTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaa < 1:1771853/70‑1 (MQ=255) tgttgtGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGGAGCCATGCGGTGTATaaaaa < 1:3667125/70‑1 (MQ=255) tgttgtGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaaaa > 1:1618931/1‑71 (MQ=255) tgttgtGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaaa < 1:347167/70‑1 (MQ=255) tgttgtGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaaa < 1:1064234/70‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTTTATAAAAAATGATCTCAt < 1:3176510/70‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCAt < 1:361600/70‑1 (MQ=255) ggCGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCAt < 1:3628710/70‑1 (MQ=255) cGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCa < 1:3401057/71‑1 (MQ=255) cGACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCa < 1:91143/71‑1 (MQ=255) gACACTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAg < 1:2663945/71‑1 (MQ=255) cTTCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATg < 1:722818/56‑1 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAg < 1:3140916/65‑1 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:3191698/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:401398/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:2687533/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1139418/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:2542739/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1244021/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:2356621/1‑71 (MQ=255) tCGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGttt > 1:1657003/1‑71 (MQ=255) cGCTTAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGAt > 1:1494214/1‑55 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:1836613/1‑63 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:722448/1‑63 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:2567014/1‑63 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGCTGTATAAAAAATGATCTCATGCAGATGTTTTGTGaa > 1:1300592/1‑71 (MQ=255) aaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAAtgt < 1:470648/71‑1 (MQ=255) cACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttg > 1:2969513/1‑71 (MQ=255) cACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttg > 1:2084346/1‑71 (MQ=255) cACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTgttg > 1:2478786/1‑71 (MQ=255) cACCAGTAGTGATTTCGCAGACATGCGGTGTATAAAAAATGAtctc > 1:1849809/1‑46 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGAt > 1:1399795/1‑35 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGAt > 1:2373016/1‑35 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAt > 1:3534386/1‑71 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAt > 1:422620/1‑71 (MQ=255) | CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACAT > W3110S.gb/344314‑344448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |