Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	381,851	A→C	77.8%	G88G (GGT→GGG)	yaiF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	381,851	0	A	C	77.8%	136.5 / 16.3	49	G88G (GGT→GGG)	yaiF	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base C (18/20);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAG  >  W3110S.gb/381790‑381912                                                              |                                                              gTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGAccc                                                            <  1:97028/65‑1 (MQ=255)  tCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATa                                                      <  1:1386314/70‑1 (MQ=255)  tCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATa                                                      <  1:2532733/70‑1 (MQ=255)          tataGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAAt                                                       <  1:2986022/61‑1 (MQ=255)            taGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt                                            >  1:2878071/1‑70 (MQ=255)            taGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt                                            >  1:2207176/1‑70 (MQ=255)               gggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACa                                        <  1:3661647/71‑1 (MQ=255)                 gCAAATCCAATTGCCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      <  1:2878031/71‑1 (MQ=255)                 gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      <  1:422902/71‑1 (MQ=255)                 gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      <  1:3122229/71‑1 (MQ=255)                 gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      <  1:3261314/71‑1 (MQ=255)                 gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      <  1:821012/71‑1 (MQ=255)                  cAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      <  1:1117612/70‑1 (MQ=255)                  cAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      >  1:579692/1‑70 (MQ=255)                  cAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      >  1:547197/1‑70 (MQ=255)                  cAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      >  1:3443192/1‑70 (MQ=255)                  cAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa                                      >  1:1853214/1‑70 (MQ=255)                     aTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATAc                                   <  1:1951870/70‑1 (MQ=255)                      tCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACgg                                 >  1:402024/1‑71 (MQ=255)                       ccAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACggg                                >  1:3385580/1‑71 (MQ=255)                       ccAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAAAACggg                                >  1:3065083/1‑71 (MQ=255)                                  tCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa                                      <  1:2317925/54‑1 (MQ=255)                                  tCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa                                      <  1:373303/54‑1 (MQ=255)                                    aGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                    >  1:2502309/1‑70 (MQ=255)                                    aGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                    >  1:34511/1‑70 (MQ=255)                                    aGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                    >  1:3266716/1‑70 (MQ=255)                                    aGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                    >  1:501033/1‑70 (MQ=255)                                    aGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGa                    >  1:101094/1‑70 (MQ=255)                                      gCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATa                                    >  1:1321422/1‑52 (MQ=255)                                      gCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATa                                    >  1:306589/1‑52 (MQ=255)                                      gCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATa                                    >  1:253865/1‑52 (MQ=255)                                      gCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATa                                    >  1:226793/1‑52 (MQ=255)                                          tttGCGCCAATCACTACCGGCCCCTGAATAACGGCAcc                                              <  1:1365220/38‑1 (MQ=255)                                              cgcCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTc          <  1:1716378/70‑1 (MQ=255)                                              cgcCAATCACTACCGGCCCCTGAATAACGGCACCATCACAAATACGGGTATTCGCGCCGATAACAACTTc          <  1:93533/70‑1 (MQ=255)                                               gcCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca               <  1:173255/64‑1 (MQ=255)                                               gcCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca               <  1:2366128/64‑1 (MQ=255)                                               gcCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca               <  1:1968386/64‑1 (MQ=255)                                                    tCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca               <  1:2590399/59‑1 (MQ=255)                                                    tCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca               <  1:1217930/59‑1 (MQ=255)                                                    tCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca               <  1:2037795/59‑1 (MQ=255)                                                     cagtACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAg  >  1:1589930/4‑71 (MQ=255)                                                     cACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAg  >  1:3000804/1‑71 (MQ=255)                                                     cACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTAGCGCCGATAACAACTTCACCTGCAg  >  1:2504602/1‑71 (MQ=255)                                                      aCTACCGGCCCCTGAATAACGGCACCATGCCAAATACGGGTATTCGCGCCGATaacaac             >  1:2891103/1‑59 (MQ=255)                                                      aCTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaac             >  1:3333339/1‑59 (MQ=255)                                                       cTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTc          <  1:699763/61‑1 (MQ=255)                                                       cTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTc          <  1:1751003/61‑1 (MQ=255)                                                         aCCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCAc        >  1:1845882/1‑61 (MQ=255)                                                              |                                                              GTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAG  >  W3110S.gb/381790‑381912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑