| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 496,170 | A→G | 100% | E609E (GAA→GAG) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 496,170 | 0 | A | G | 83.3% | 56.3 / 4.9 | 24 | E609E (GAA→GAG) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base G (10/10); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAT > W3110S.gb/496114‑496231 | gAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGtt < 1:1975708/71‑1 (MQ=255)gAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGtt < 1:1906714/71‑1 (MQ=255) tAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATtcgt > 1:2798431/1‑71 (MQ=255) tAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGAt > 1:1866532/1‑53 (MQ=255) gAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTAtt > 1:206421/1‑66 (MQ=255) ctgctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTggagga > 1:1596602/1‑47 (MQ=255) ctgctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTAt > 1:1000782/1‑71 (MQ=255) ctgctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTAt > 1:1827311/1‑71 (MQ=255) ctgctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTAt > 1:1649855/1‑71 (MQ=255) gctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAAc > 1:882590/1‑52 (MQ=255) gctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAAc > 1:1396076/1‑52 (MQ=255) gctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAAc > 1:2397696/1‑52 (MQ=255) gctgctgGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAGGATCCGAAc > 1:783216/1‑52 (MQ=255) gctgctGGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGGCGTATGAACCAGCTGCTGGtt < 1:332490/70‑1 (MQ=255) ctgGCAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTg < 1:2910526/71‑1 (MQ=255) cAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:2380480/71‑1 (MQ=255) cAGAACGCGGCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:503890/71‑1 (MQ=255) cAGAACGCGGCACCCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:353572/71‑1 (MQ=255) gCACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGt < 1:2841976/62‑1 (MQ=255) cACGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAt > 1:1241029/1‑71 (MQ=255) cGCTGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGtt < 1:1635320/50‑1 (MQ=255) cGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGtt < 1:426270/50‑1 (MQ=255) cGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGtt < 1:3103366/50‑1 (MQ=255) cGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGtt < 1:910661/50‑1 (MQ=255) tGGAGGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGtt < 1:30969/47‑1 (MQ=255) | GAGTGGGTAGAACTGCTGCTGGATCAGGCGCTGCTGGCAGAACGCGGCACGCTGGAAGATCCGAACCTGTTTATTCGTCGTATGAACCAGCTGCTGGTTTCCTGATGTAATGCCGGAT > W3110S.gb/496114‑496231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |