| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 515,934 | C→T | 82.0% | A43V (GCG→GTG) | ybbM → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 515,934 | 0 | C | T | 82.0% | 111.2 / 18.6 | 50 | A43V (GCG→GTG) | ybbM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (4/5); new base T (27/14); total (31/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAGCCT > W3110S.gb/515867‑516000 | gCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGAt < 1:851049/71‑1 (MQ=255) cTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt > 1:2818992/1‑70 (MQ=255) cTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt > 1:1276036/1‑70 (MQ=255) cTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt > 1:2035987/1‑70 (MQ=255) cTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAt > 1:3552070/1‑69 (MQ=255) ttAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt > 1:1996516/1‑69 (MQ=255) ttAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAAtt > 1:2568521/1‑69 (MQ=255) gCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:2011793/71‑1 (MQ=255) gCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:2777215/71‑1 (MQ=255) gCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt < 1:2246246/71‑1 (MQ=255) gCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGatt < 1:1054657/71‑1 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:956864/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTc > 1:2009641/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTc > 1:2018235/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTc > 1:594624/1‑71 (MQ=255) aaGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTc > 1:3427165/1‑71 (MQ=255) gAAAAACTGGCGCTGGAGAAAGATATTCTCTTGAGCGTCGGGCGAGTGATAATTCAGCTGattatt < 1:1675236/66‑1 (MQ=255) gAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGattatt < 1:194625/66‑1 (MQ=255) gAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGattatt < 1:1773226/66‑1 (MQ=255) gAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGattatt < 1:544190/66‑1 (MQ=255) aaaaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGattatt < 1:2610911/65‑1 (MQ=255) aaaCTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTa > 1:1357559/1‑71 (MQ=255) ggCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat > 1:1005638/1‑54 (MQ=255) cTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGa < 1:929919/49‑1 (MQ=255) ggAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGatt > 1:2943779/1‑49 (MQ=255) ggAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGat > 1:2831390/1‑48 (MQ=255) ggAGAAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAg > 1:299073/1‑70 (MQ=255) ggAGAAAGATATTCTCTGGAGCGTCGCGCGAGTGATAATTCAGCTGatt > 1:53609/1‑49 (MQ=255) agaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta > 1:177839/1‑70 (MQ=255) agaAAGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGta > 1:314989/1‑70 (MQ=255) gaAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGtata < 1:2603071/71‑1 (MQ=255) aaGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtt < 1:2658815/71‑1 (MQ=255) aaGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtt < 1:860644/71‑1 (MQ=255) aaGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtt < 1:1936979/71‑1 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTAt > 1:1064632/1‑55 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:995672/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAttt > 1:3443216/1‑71 (MQ=255) aGATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtt > 1:2541516/1‑70 (MQ=255) gATATTCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATAtttt < 1:145323/71‑1 (MQ=255) ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGaa > 1:758598/1‑58 (MQ=255) ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGaa > 1:3144933/1‑58 (MQ=255) ttCTCTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGc > 1:1780015/1‑71 (MQ=255) ttCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTAtg > 1:3498935/1‑51 (MQ=255) tctcTGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCg > 1:1345194/1‑71 (MQ=255) ctctGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTc > 1:2551128/1‑66 (MQ=255) tGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:868707/69‑1 (MQ=255) tGGAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTg < 1:1101706/69‑1 (MQ=255) gAGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgat < 1:2935727/70‑1 (MQ=255) aGCGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGgatga > 1:1474517/1‑71 (MQ=255) cGTCGGGCGAGTGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGatgatg > 1:681802/1‑71 (MQ=255) cgagcgaTAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAGCCt > 1:1391382/1‑71 (MQ=255) | GCAATCTTAATTAGCCATAAAGAAAAACTGGCGCTGGAGAAAGATATTCTCTGGAGCGTCGGGCGAGCGATAATTCAGCTGATTATTGTCGGCTATGTGCTGAAGTATATTTTCAGCGTGGATGATGCCAGCCT > W3110S.gb/515867‑516000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |