| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 531,446 | C→T | 56.1% | intergenic (‑1/‑229) | ybbS ← / → allA | transcriptional activator of the allD operon/ureidoglycolate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 531,446 | 0 | C | T | 56.1% | 9.0 / 52.7 | 41 | intergenic (‑1/‑229) | ybbS/allA | transcriptional activator of the allD operon/ureidoglycolate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (6/12); new base T (5/18); total (11/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
CGCTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTA > W3110S.gb/531380‑531514 | cGCTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCa < 1:1427187/71‑1 (MQ=255) cTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAgg < 1:647063/71‑1 (MQ=255) cTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAgg < 1:1878352/71‑1 (MQ=255) cTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAgg < 1:3543929/71‑1 (MQ=255) cTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAgg < 1:2317764/71‑1 (MQ=255) ttGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGt < 1:1160998/69‑1 (MQ=255) ttGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGt < 1:2088048/69‑1 (MQ=255) ccTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTa < 1:468384/69‑1 (MQ=255) ttCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCg > 1:765590/1‑70 (MQ=255) ttCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCg > 1:1749263/1‑70 (MQ=255) cAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCa < 1:2900811/70‑1 (MQ=255) aGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa < 1:1239005/70‑1 (MQ=255) aGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa < 1:1232007/70‑1 (MQ=255) aGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa < 1:2832313/70‑1 (MQ=255) ccGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCa < 1:1685002/42‑1 (MQ=255) ccGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCa < 1:2965180/42‑1 (MQ=255) cGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACAtttt > 1:388959/1‑39 (MQ=255) cGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACAtttt > 1:111716/1‑39 (MQ=255) cAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGa < 1:2977052/71‑1 (MQ=255) aaTGAAAGTCCGCAAGGTTTCTGGATCGAACAtttt < 1:2532336/36‑1 (MQ=255) aaTGAAAGTCCGCAAGGTTTCTGGATCGAACAtttt < 1:1700478/36‑1 (MQ=255) aaTAAAAGTCCGCAAGGTTTCTGGATCGAACAtttt < 1:1363139/36‑1 (MQ=37) tGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATcc < 1:2604631/43‑1 (MQ=255) tGAAAGTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATcc < 1:115119/43‑1 (MQ=255) gTCCGCAAGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:1583186/71‑1 (MQ=255) tCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTAt < 1:2736954/71‑1 (MQ=255) ccGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATc > 1:2394048/1‑71 (MQ=255) cGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATc < 1:124420/70‑1 (MQ=255) aGGTTTCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCctct > 1:2873133/1‑70 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:2818720/61‑1 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:2780995/61‑1 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:2502292/61‑1 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:395393/61‑1 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGAtt < 1:166331/71‑1 (MQ=255) tttCTGGATCGAACATTTTCAGGTATCCCCTTTTAAATCCGCAAATTGCGTGATTTTCTTATCCTCTGAtt < 1:1432415/71‑1 (MQ=255) aTCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGt < 1:1742124/71‑1 (MQ=255) gAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAtt > 1:2785666/1‑71 (MQ=255) gAACATTTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAtt > 1:225090/1‑71 (MQ=255) cATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:3123540/48‑1 (MQ=255) cATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTa < 1:331030/48‑1 (MQ=255) cATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAtttt > 1:1847084/1‑70 (MQ=255) cATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAtttt > 1:2977800/1‑70 (MQ=255) cATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAtttt > 1:2030415/1‑70 (MQ=255) cATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTAtttt > 1:2484459/1‑70 (MQ=255) aTCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTa < 1:3644304/71‑1 (MQ=255) tCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTa > 1:1986909/1‑70 (MQ=255) | CGCTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTCTGATTTATCAGTATTTTTA > W3110S.gb/531380‑531514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |